Enantioselective Effects of Methamidophos on the Coelomocytes Lysosomal Membrane Stability of Eisenia fetida
Enantioselective Effects of Methamidophos on the Coelomocytes Lysosomal Membrane Stability of Eisenia fetida
复制标题
甲胺磷对赤子爱胜蚓体腔细胞溶酶体膜稳定性的影响
DOI：
10.1007/s00128-012-0861-y
复制
发表时间：
2012-10
影响因子：
2.7
通讯作者：
Ma, Yun
中科院分区：
文献类型：
--
作者：
Chen, Linhua;Lu, Xianting;Ma, Yun
许多有机磷杀虫剂都是手性化合物。研究了有机磷杀虫剂甲胺磷对蚯蚓体腔细胞溶酶体膜稳定性的影响：（1）采用高效液相色谱法，以商品手性柱为分离柱，对甲胺磷对映体进行了完全分离;（2）采用中性红保留法对溶酶体膜稳定性进行了评价。结果表明，随着浓度的增加，甲胺磷的单个立体异构体和外消旋体对溶酶体膜的破坏作用明显。中性红的保留时间显着下降，从76.88至29.78分钟。（+）-和（-）-甲胺磷表现出更容易破坏溶酶体膜的完整性比外消旋体。然而，立体异构体之间的差异影响很小。
Many of organophosphorous insecticides are chiral compounds. In this study, the enantioselective effects of organophosphate insecticide methamidophos on the coelomocytes lysosomal membrane stability of earthworm Eisenia fetida were studied: (1) The enantiomers of methamidophos were absolutely separated by high-performance liquid chromatography with a commercial chiral column; (2) The neutral red retention assay was used to judge the lysosomal membrane stability. The results showed that with the concentration increasing, lysosomal membranes have been significantly destroyed by individual stereoisomers and racemate of methamidophos. The neutral red retention times were significantly descended from 76.88 to 29.78 min. Both (+)- and (-)-methamidophos showed more prone to destroy the integrity of the lysosomal membrane than the racemate. However, the different effect between stereoisomers is slight.
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DOI：
10.4324/9780203875773
发表时间：
2009-05
期刊：
--
影响因子：
--
作者：
Richard Woodward
通讯作者：
Richard Woodward
DOI：
--
发表时间：
2003
期刊：
--
影响因子：
--
作者：
Liaomin;Xiesiaomei;A.Subhani
通讯作者：
Liaomin;Xiesiaomei;A.Subhani
影响因子：
3.3
作者：
M. Moore;J. Allen;A. McVeigh;A. McVeigh
通讯作者：
M. Moore;J. Allen;A. McVeigh;A. McVeigh
影响因子：
5.8
作者：
C. Svendsen;J. Weeks
通讯作者：
C. Svendsen;J. Weeks
影响因子：
8.8
作者：
Yingtao Yu;Qixing Zhou
通讯作者：
Yingtao Yu;Qixing Zhou