Pressure-dependent structure changes in barnase on ligand binding reveal intermediate rate fluctuations.
Pressure-dependent structure changes in barnase on ligand binding reveal intermediate rate fluctuations.
复制标题
配体结合时 Barnase 的压力依赖性结构变化揭示了中等速率波动。
DOI：
10.1016/j.bpj.2009.06.022
复制
发表时间：
2009
影响因子：
3.4
通讯作者：
Wilton DJ
中科院分区：
文献类型：
--
作者：
Wilton DJ
In this work we measured1H NMR chemical shifts for the ribonuclease barnase at pressures from 3 MPa to 200 MPa, both free and bound to d(CGAC). Shift changes with pressure were used as restraints to determine the change in structure with pressure. Free barnase is compressed by ∼0.7%. The largest changes are on the ligand-binding face close to Lys-27, which is the recognition site for the cleaved phosphate bond. This part of the protein also contains the buried water molecules. In the presence of d(CGAC), the compressibility is reduced by ∼70% and the region of structural change is altered: the ligand-binding face is now almost incompressible, whereas changes occur at the opposite face. Because compressibility is proportional to mean square volume fluctuation, we conclude that in free barnase, volume fluctuation is largest close to the active site, but when the inhibitor is bound, the fluctuations become much smaller and are located mainly on the opposite face. The timescale of the fluctuations is nanoseconds to microseconds, consistent with the degree of ordering required for the fluctuations, which are intermediate between rapid uncorrelated side-chain dynamics and slow conformational transitions. The high-pressure technique is therefore useful for characterizing motions on this relatively inaccessible timescale.
登录
查看更多内容
影响因子：
5.6
作者：
M. Refaee;T. Tezuka;K. Akasaka;M. Williamson
通讯作者：
M. Refaee;T. Tezuka;K. Akasaka;M. Williamson
核苷酸构象平衡的变化有助于提高 Barnase 中 GpAp 相对于 GpA 的催化速率
DOI：
--
发表时间：
2004
期刊：
Proteins: Structure, Function, and Bioinformatics
影响因子：
--
作者：
J. Giraldo;L. De Maria;S. Wodak
通讯作者：
S. Wodak
DOI：
10.1016/s0167-4838(00)00019-4
发表时间：
2000-05-23
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
影响因子：
--
作者：
Kamiyama, T;Gekko, K
通讯作者：
Gekko, K
DOI：
--
发表时间：
1989
期刊：
影响因子：
--
作者：
K. Gekko;Y. Hasegawa
通讯作者：
Y. Hasegawa
蛋白质的高分辨率核磁共振研究。
DOI：
10.1016/s0167-4838(01)00341-7
发表时间：
2002
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
J. Jonas
通讯作者：
J. Jonas