Pseudacidovorax intermedius NH-1, a novel marine nitrogen-fixing bacterium isolated from the South China Sea
Pseudacidovorax intermedius NH-1, a novel marine nitrogen-fixing bacterium isolated from the South China Sea
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Pseudaacidovorax intermedius NH-1，一种从南海分离的新型海洋固氮细菌
DOI：
10.1007/s11274-012-1093-3
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发表时间：
2012
影响因子：
4.1
通讯作者：
San
中科院分区：
文献类型：
--
作者：
Li;San
对从南海表层沉积物中分离到的一株新的固氮菌NH-1进行了分类学和遗传学研究。通过PCR扩增确定nifH基因的存在。根据表型特征、16 S rDNA序列测定、G+C含量测定和DNA-DNA杂交结果，将NH-1菌株鉴定为假食酸菌属（Pseudacidovorax）。分离物NH-1经鉴定为中间假食酸菌（Pseudacidovorax intermedius）。此外，我们还研究了环境因素与NH-1固氮酶活性之间的联系。我们发现，NH-1的固氮能力强烈变化时，细胞生长与不同的铵离子和氧浓度，氨基酸和碳水化合物。这是首次从海洋中分离到假食酸菌，也是首次研究不同培养条件对该菌固氮酶活性的影响。这项研究提供的证据表明，海洋固氮微生物远比目前认识到的更加多样化。
We have taxonomically and phylogenetically characterized a novel nitrogen-fixing bacterial strain, NH-1, which was recently isolated from surface sediments of the South China Sea. The presence of the nifH gene was determined by PCR amplification. The strain NH-1 was found to belong to the genus Pseudacidovorax based on phenotypic characterization, 16S rDNA sequencing, G+C content and DNA–DNA hybridization. Isolate NH-1 was identified as Pseudacidovorax intermedius. In addition, we investigated the links between environmental factors and the nitrogenase activity of NH-1. We found that the nitrogen fixation capacity of NH-1 varied strongly when cells were grown with different ammonium ion and oxygen concentrations, amino acids and carbohydrates. This is the first report of the isolation of Pseudacidovorax from the ocean and the first study to explore the effects of different culture conditions on the nitrogenase activities of the isolate. This study provides evidence that marine nitrogen-fixing microorganisms are far more diverse than currently recognized.
影响因子：
11
作者：
Moisander, Pia H.;Beinart, Roxanne A.;Zehr, Jonathan P.
通讯作者：
Zehr, Jonathan P.
影响因子：
2.7
作者：
M. Hofmann;David A. Brian
通讯作者：
M. Hofmann;David A. Brian