Use of intercellular proximity labeling to quantify and decipher cell-cell interactions directed by diversified molecular pairs.
Use of intercellular proximity labeling to quantify and decipher cell-cell interactions directed by diversified molecular pairs.
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DOI：
10.1126/sciadv.add2337
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发表时间：
2022-12-21
期刊：
影响因子：
13.6
通讯作者：
中科院分区：
文献类型：
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作者：
FucoID is an intercellular proximity labeling technique for studying cell-cell interactions (CCIs) via fucosyltransferase (FT)–meditated fucosyl-biotinylation, which has been applied to probe antigen-specific dendritic cell (DC)–T cell interactions. In this system, bait cells of interest with cell surface–anchored FT are used to capture the interacting prey cells by transferring a biotin-modified substrate to prey cells. Here, we leveraged FucoID to study CCIs directed by different molecular pairs, e.g., programmed cell death protein-1(PD-1)/programmed cell death protein-ligand-1 (PD-L1), and identify unknown or little studied CCIs, e.g., the interaction of DCs and B cells. To expand the application of FucoID to complex systems, we also synthesized site-specific antibody-based FT conjugate, which substantially improves the ability of FucoID to probe molecular signatures of specific CCI when cells of interest (bait cells) cannot be purified, e.g., in clinical samples. Collectively, these studies demonstrate the general applicability of FucoID to study unknown CCIs in complex systems at a molecular resolution. FucoID is a platform that can be used to discover and quantify cell-cell interactions mediated by diverse molecular pairs.
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影响因子：
46.9
作者：
Chua, Robert Lorenz;Lukassen, Soeren;Eils, Roland
通讯作者：
Eils, Roland
DOI：
10.1158/1078-0432.ccr-20-3770
发表时间：
2021-10-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
通讯作者：
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DOI：
10.1038/nri.2017.74
发表时间：
2017-11
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
Huse M
通讯作者：
Huse M
影响因子：
14.8
作者：
Efremova, Mirjana;Vento-Tormo, Miquel;Vento-Tormo, Roser
通讯作者：
Vento-Tormo, Roser
影响因子：
4.3
作者：
Cain, Margo P.;Hernandez, Belinda J.;Chen, Jichao
通讯作者：
Chen, Jichao