The virulence phenotypes and molecular epidemiological characteristics of Vibrio fluvialis in China.
The virulence phenotypes and molecular epidemiological characteristics of Vibrio fluvialis in China.
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中国河流弧菌毒力表型及分子流行病学特征
DOI：
10.1186/1757-4749-5-6
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发表时间：
2013-03-22
期刊：
影响因子：
4.2
通讯作者：
Liang W
中科院分区：
文献类型：
--
作者：
Liang P;Cui X;Du X;Kan B;Liang W
背景：河流弧菌被认为是一种新出现的食源性致病菌，在世界各地，特别是在发展中国家的沿海地区和卫生条件较差的地区，已成为高度危害人类公共健康的疾病。中国分离的河弧菌毒力因子分布、微生物学及分子流行病学特征有待进一步研究。方法与结果：针对河弧菌毒力决定因素进行聚合酶链式反应，并进行代谢、毒力及药敏等表型试验。采用脉冲场凝胶电泳法(PFGE)分析菌株间的亲缘关系。首次报道了一株精氨酸二水解酶系统缺失的菌株，并用聚合酶链式反应从分子水平上证实了该菌株的存在。所有菌株均检测到毒力基因vfh、huPo和vfpA，不同菌株产生溶血素、胞毒、蛋白酶和生物被膜的能力不同。对β-内酰胺类、阿奇霉素和磺胺甲恶唑耐药率较高。27%的测试菌株对两种和三种抗生素具有抗药性。脉冲场凝胶电泳法分析表明，河弧菌具有较大的遗传异质性。结论：本研究首次对中国地区河弧菌的生物学特性和分子流行病学特征进行了评价。发现了一些不常见的生化特征。毒力基因在患者来源和水产品来源的分离株中分布广泛，且毒力表型的出现因菌株而异。今后需要继续和加强以实验室为基础的监测，同时系统地收集病例或暴发的流行病学信息。
Background:Vibrio fluvialis is considered to be an emerging foodborne pathogen and has been becoming a high human public health hazard all over the world, especially in coastal areas of developing countries and regions with poor sanitation. The distribution of virulence factors, microbiological and molecular epidemiological features of V. fluvialis isolates in China remains to be examined.Methods and results:PCR targeted at the virulence determinants and phenotype tests including metabolism, virulence and antibiotic susceptibility were performed. Pulsed-field gel electrophoresis (PFGE) analysis was used to access the relatedness of isolates. A strain with deletion of the arginine dihydrolase system was first reported and proved in molecular level by PCR. Virulence genes vfh, hupO and vfpA were detected in all strains, the ability to produce hemolysin, cytotxin, protease and biofilm formation varied with strains. High resistance rate to β-lactams, azithromycin and sulfamethoxazole were observed. Twenty-seven percent of test strains showed resistant to two and three antibiotics. PFGE analysis demonstrated great genetic heterogeneity of test V. fluvialis strains.Conclusion:This study evaluated firstly the biological characteristics and molecular epidemiological features of V. fluvialis in China. Some uncommon biochemical characteristics were found. Virulence genes were widely distributed in the isolates from patient and seafood sources, and the occurrence of virulence phenotypes varied with strains. Continued and enhanced laboratory based-surveillance is needed in the future together with systematically collection of the epidemiological information of the cases or the outbreaks.
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DOI：
10.3390/ijerph7103628
发表时间：
2010-10
影响因子：
--
作者：
Igbinosa EO;Okoh AI
通讯作者：
Okoh AI
影响因子：
4.9
作者：
Hochhut, B;Lotfi, Y;Waldor, MK
通讯作者：
Waldor, MK
DOI：
10.1016/j.ijantimicag.2011.04.013
发表时间：
2011-08-01
影响因子：
10.8
作者：
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通讯作者：
Ramamurthy, T.
影响因子：
6.7
作者：
Amel, Ben Kahla-Nakbi;Amine, Besbes;Amina, Bakhrouf
通讯作者：
Amina, Bakhrouf
影响因子：
5.2
作者：
Ahmed, AM;Nakagawa, T;Shimamoto, T
通讯作者：
Shimamoto, T