Comparison of two probe designs for determining intraocular oxygen distribution.
Comparison of two probe designs for determining intraocular oxygen distribution.
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DOI：
10.1136/bjo.2010.186064
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发表时间：
2011-01
期刊：
影响因子：
--
通讯作者：
Beebe DC
中科院分区：
文献类型：
--
作者：
Park YH;Shui YB;Beebe DC
眼内氧水平的改变是眼部疾病的重要诱因或指征。极谱电极和光纤传感器（光极）已被用于测量氧气和绘制动物模型和人眼中氧气的分布。最近的一项研究报告了使用商业电极来比较接受与视网膜中央静脉阻塞、黄斑裂孔或视网膜前膜相关的玻璃体切除术的患者的玻璃体中的氧分布。这项研究的结果是在与以前的措施，在人类玻璃体中的氧分布，使用极谱或光学传感器的差异。为了解决这一差异，本研究比较了在体外或在动物眼睛中进行的测量，使用在以前的研究中采用的电极或不同设计的光纤传感器。比较体外和体内测量。在体外，两种器械报告了相似的氧气水平，尽管电极始终检测到高于计算值的水平。在兔子眼中，电极的响应时间很慢，无法检测到氧气梯度，而氧气梯度很容易通过较小的光电管测量。当电极插入与人眼大小相似的眼睛时，参考热敏电阻测量的是眼睛外部的温度，而不是玻璃体中的温度。在先前的研究中使用的电极的设计使得其不适合于测量眼睛中的氧气分布。
Alterations in intraocular oxygen levels are important contributors to, or indications of ocular disease. Polarographic electrodes and fiberoptic sensors (optodes) have been used to measure oxygen and to map the distribution of oxygen in animal models and in human eyes. A recent study reported the use of a commercial electrode to compare oxygen distribution in the vitreous of patients undergoing vitrectomy related to central retinal vein occlusion, macular hole or preretinal membrane. The results of this study were at variance with previous measures of oxygen distribution in the human vitreous using polarographic or optical sensors. To resolve this discrepancy, the present study compared measurements made in vitro or in animal eyes, using the electrode employed in the previous study or a fiberoptic sensor of different design. Comparative in vitro and in vivo measurements. In vitro, the two devices reported similar levels of oxygen, although the electrode consistently detected levels above the calculated values. In rabbit eyes, the electrode had a slow response time and was unable to detect oxygen gradients that were readily measured by the smaller optode. When the electrode was inserted into an eye of similar size to the human eye, the reference thermistor measured the temperature outside the eye, not in the vitreous. The design of the electrode used in the previous study makes it unsuitable for measurements of oxygen distribution in the eye.
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影响因子：
4.2
作者：
Chang, Stanley
通讯作者：
Chang, Stanley
DOI：
10.1007/s00417-009-1072-z
发表时间：
2009-08-01
影响因子：
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4.2
作者：
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影响因子：
3.3
作者：
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