Tryptophan-based fluorophores for studying protein conformational changes.
Tryptophan-based fluorophores for studying protein conformational changes.
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DOI：
10.1016/j.bmc.2014.09.015
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发表时间：
2014-11-01
影响因子：
3.5
通讯作者：
Hecht SM
中科院分区：
文献类型：
--
作者：
Talukder P;Chen S;Liu CT;Baldwin EA;Benkovic SJ;Hecht SM
With the continuing interest in deciphering the interplay between protein function and conformational changes, small fluorescence probes will be especially useful for tracking changes in the crowded protein interior space. Presently, we describe the potential utility of six unnatural amino acid fluorescence donors structurally related to tryptophan and show how they can be efficiently incorporated into a protein as fluorescence probes. We also examine the various photophysical properties of the new Trp analogues, which are significantly redshifted in their fluorescence spectra relative to tryptophan. In general, the Trp analogues were well tolerated when inserted into E. coli DHFR, and did not perturb enzyme activity, although substitution for Trp22 did result in a diminution in DHFR activity. Further, it was demonstrated that D and E at position 37 formed efficient FRET pairs with acridon-2-ylalanine (Acd) at position 17. The same was also true for a DHFR construct containing E at position 79 and Acd at position 17. Together, these findings demonstrate that these tryptophan analogues can be introduced into DHFR with minimal disruption of function, and that they can be employed for the selective study of targeted conformational or electrostatic changes in proteins, even in the presence of unmodified tryptophans.
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DOI：
10.1126/science.1198542
发表时间：
2011-04-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bhabha G;Lee J;Ekiert DC;Gam J;Wilson IA;Dyson HJ;Benkovic SJ;Wright PE
通讯作者：
Wright PE
影响因子：
4.1
作者：
Johansson, Staffan;Redeby, Theres;Borje, Anna
通讯作者：
Borje, Anna
影响因子：
3.5
作者：
Hamada, H;Kameshima, N;Sisido, M
通讯作者：
Sisido, M
影响因子：
2.9
作者：
EDELHOCH, H
通讯作者：
EDELHOCH, H
DOI：
10.1139/v03-035
发表时间：
2003-06-01
期刊：
CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE
影响因子：
--
作者：
Gibson, G;Neverov, AA;Brown, RS
通讯作者：
Brown, RS