Aptamer-Based Imaging of Polyisoprenoids in the Malaria Parasite.
Aptamer-Based Imaging of Polyisoprenoids in the Malaria Parasite.
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DOI：
10.3390/molecules29010178
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发表时间：
2023-12-28
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影响因子：
--
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中科院分区：
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Dolichol是甲羟戊酸和2C-甲基-D-β-4-磷酸途径的类异戊二烯终产物。通过将几个分子的异戊烯基二磷酸添加到法呢基二磷酸来引发长萜醇的合成。该反应由顺式异戊二烯基转移酶催化，并导致聚异戊二烯基二磷酸的形成。随后的步骤包括通过聚戊烯醇还原酶对α-异戊二烯单元进行脱磷酸化和还原，从而产生多萜醇。长萜醇的大小取决于结合的异戊二烯单元的数量而变化。在真核生物中，长萜醇被合成为四种或更多种不同长度的混合物。它们的生物合成被预测发生在内质网中，其中长萜醇在蛋白质糖基化中起重要作用。在这项研究中，我们已经开发了一种选择的适体靶向dolichols和增强其特异性，通过纳入脂肪酸的负选择。一种适体显示出对α-异戊二烯单元中含有至少一个氧原子的线性聚类异戊二烯（如醇或醛）的高度富集和特异性。选择的适体被证明是一个有价值的工具，在疟原虫的聚异戊二烯的亚细胞定位。据我们所知，这是第一次使用基于适体的成像技术将聚类异戊二烯定位在细胞内。
Dolichols are isoprenoid end-products of the mevalonate and 2C-methyl-D-erythritol-4-phosphate pathways. The synthesis of dolichols is initiated with the addition of several molecules of isopentenyl diphosphate to farnesyl diphosphate. This reaction is catalyzed by a cis-prenyltransferase and leads to the formation of polyprenyl diphosphate. Subsequent steps involve the dephosphorylation and reduction of the α-isoprene unit by a polyprenol reductase, resulting in the generation of dolichol. The size of the dolichol varies, depending on the number of isoprene units incorporated. In eukaryotes, dolichols are synthesized as a mixture of four or more different lengths. Their biosynthesis is predicted to occur in the endoplasmic reticulum, where dolichols play an essential role in protein glycosylation. In this study, we have developed a selection of aptamers targeting dolichols and enhanced their specificity by incorporating fatty acids for negative selection. One aptamer showed high enrichment and specificity for linear polyisoprenoids containing at least one oxygen atom, such as an alcohol or aldehyde, in the α-isoprene unit. The selected aptamer proved to be a valuable tool for the subcellular localization of polyisoprenoids in the malaria parasite. To the best of our knowledge, this is the first time that polyisoprenoids have been localized within a cell using aptamer-based imaging techniques.
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影响因子：
4.2
作者：
Cantagrel, Vincent;Lefeber, Dirk J.
通讯作者：
Lefeber, Dirk J.
DOI：
10.1007/978-1-4939-6857-2_16
发表时间：
2017-01-01
期刊：
SYNTHETIC ANTIBODIES
影响因子：
--
作者：
Nam Nguyen Quang;Miodek, Anna;Duconge, Frederic
通讯作者：
Duconge, Frederic
影响因子：
14.9
作者：
CORPET, F
通讯作者：
CORPET, F
DOI：
10.3390/molecules28093728
发表时间：
2023-04-26
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Kara N;Ayoub N;Ilgu H;Fotiadis D;Ilgu M
通讯作者：
Ilgu M
影响因子：
1.9
作者：
Komaszylo Née Siedlecka J;Kania M;Masnyk M;Cmoch P;Lozinska I;Czarnocki Z;Skorupinska-Tudek K;Danikiewicz W;Swiezewska E
通讯作者：
Swiezewska E