Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications.
Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications.
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DOI：
10.3390/molecules28093728
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发表时间：
2023-04-26
期刊：
影响因子：
--
通讯作者：
Ilgu M
中科院分区：
文献类型：
--
作者：
Kara N;Ayoub N;Ilgu H;Fotiadis D;Ilgu M
许多生物过程（生理或病理）都与膜蛋白（MPs）有关，它几乎占人类蛋白质总数的30%。因此，MPs可以作为疾病诊断和预后的预测性分子生物标志物。事实上，细胞表面MPs是目前用于疾病检测的处方治疗药物和诊断分子的一类重要的有吸引力的靶标。被称为适体的寡核苷酸可以通过体外文库进化的迭代轮次来选择具有高亲和力和选择性的特定靶标，称为配体的指数富集系统进化（SELEX）。作为抗体的替代品，适体具有热稳定性、低成本、可重复使用、易于化学修饰以及与各种检测技术兼容等独特特性。特别是，固定化适体传感平台已经被用于诊断研究，并且与其他分析技术相比，已经证明了显著的价值。这些“适体传感器”根据其工作原理可以分为几种类型，通常是电化学、光学或质量敏感的。在这篇综述中，我们回顾了基于适配体的mp传感技术在诊断应用方面的研究，并包括近年来开展的新方法变化。
Many biological processes (physiological or pathological) are relevant to membrane proteins (MPs), which account for almost 30% of the total of human proteins. As such, MPs can serve as predictive molecular biomarkers for disease diagnosis and prognosis. Indeed, cell surface MPs are an important class of attractive targets of the currently prescribed therapeutic drugs and diagnostic molecules used in disease detection. The oligonucleotides known as aptamers can be selected against a particular target with high affinity and selectivity by iterative rounds of in vitro library evolution, known as Systematic Evolution of Ligands by EXponential Enrichment (SELEX). As an alternative to antibodies, aptamers offer unique features like thermal stability, low-cost, reuse, ease of chemical modification, and compatibility with various detection techniques. Particularly, immobilized-aptamer sensing platforms have been under investigation for diagnostics and have demonstrated significant value compared to other analytical techniques. These “aptasensors” can be classified into several types based on their working principle, which are commonly electrochemical, optical, or mass-sensitive. In this review, we review the studies on aptamer-based MP-sensing technologies for diagnostic applications and have included new methodological variations undertaken in recent years.
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DOI：
10.1038/mtna.2016.73
发表时间：
2016-09-20
期刊：
Molecular therapy. Nucleic acids
影响因子：
--
作者：
通讯作者：
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影响因子：
6.4
作者：
Sefah, Kwame;Bae, Kyung-Mi;Phillips, Joseph A.;Siemann, Dietmar W.;Su, Zhen;McClellan, Steve;Vieweg, Johannes;Tan, Weihong
通讯作者：
Tan, Weihong
影响因子：
8.8
作者：
Affinito, Alessandra;Quintavalle, Cristina;Condorelli, Gerolama
通讯作者：
Condorelli, Gerolama
影响因子：
5.4
作者：
Almen, Markus Sallman;Nordstrom, Karl J. V.;Schioth, Helgi B.
通讯作者：
Schioth, Helgi B.
影响因子：
3.1
作者：
Bahmani, Amin;Shokri, Ehsan;Hosseinkhani, Saman
通讯作者：
Hosseinkhani, Saman