Smart-phone based computational microscopy using multi-frame contact imaging on a fiber-optic array.
Smart-phone based computational microscopy using multi-frame contact imaging on a fiber-optic array.
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DOI：
10.1039/c3lc50589h
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发表时间：
2013-10-21
期刊：
影响因子：
6.1
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Navruz I;Coskun AF;Wong J;Mohammad S;Tseng D;Nagi R;Phillips S;Ozcan A
我们展示了一种基于手机的接触式显微镜平台，称为接触式显微镜，它可以在传输模式下对高密度或连接的样品进行成像。这款轻便紧凑的显微镜重约76克，使用光学机械附件安装在手机现有的摄像单元上，感兴趣的平面样品与锥形光纤阵列的顶面接触。这种基于玻璃的锥形光纤阵列在其顶面具有比底面高9倍的光缆密度，并由非相干光源照明，例如简单的发光二极管(∼)。然后通过该光纤电缆阵列对穿过物体的透射光图案进行采样，将样品的传输图像传输到锥度的另一侧，每个方向的∼为3倍。这个物体的放大图像位于光纤阵列的底面，然后使用两个镜头投影到手机的cmos图像传感器上。在将样品和手机摄像头保持在固定位置的同时，然后以例如1-2度的离散角度增量手动旋转光纤阵列。在光纤阵列的每个角度位置，使用手机摄像头捕捉接触图像，为同一样本创建一系列传输图像。这些多帧图像通过智能手机上运行的定制开发的Android应用程序，基于移位相加算法被数字融合在一起，提供通过手机屏幕可视化的最终样本显微图像。这个最后的计算步骤提高了分辨率，并且还消除了由于光纤阵列表面的传输强度的不均匀采样而产生的空间伪影。我们通过成像分辨率测试图表和血液涂片验证了这种基于手机的接触式示波器的性能。
We demonstrate a cellphone based contact microscopy platform, termed Contact Scope, which can image highly dense or connected samples in transmission mode. Weighing approximately 76 grams, this portable and compact microscope is installed on the existing camera unit of a cellphone using an opto-mechanical add-on, where planar samples of interest are placed in contact with the top facet of a tapered fiber-optic array. This glass-based tapered fiber array has ∼9 fold higher density of fiber optic cables on its top facet compared to the bottom one and is illuminated by an incoherent light source, e.g., a simple light-emitting-diode (LED). The transmitted light pattern through the object is then sampled by this array of fiber optic cables, delivering a transmission image of the sample onto the other side of the taper, with ∼3× magnification in each direction. This magnified image of the object, located at the bottom facet of the fiber array, is then projected onto the CMOS image sensor of the cellphone using two lenses. While keeping the sample and the cellphone camera at a fixed position, the fiber-optic array is then manually rotated with discrete angular increments of e.g., 1-2 degrees. At each angular position of the fiber-optic array, contact images are captured using the cellphone camera, creating a sequence of transmission images for the same sample. These multi-frame images are digitally fused together based on a shift-and-add algorithm through a custom-developed Android application running on the smart-phone, providing the final microscopic image of the sample, visualized through the screen of the phone. This final computation step improves the resolution and also gets rid of spatial artefacts that arise due to non-uniform sampling of the transmission intensity at the fiber optic array surface. We validated the performance of this cellphone based Contact Scope by imaging resolution test charts and blood smears.
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影响因子：
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