Response to excess copper in the hyperthermophile Sulfolobus solfataricus strain 98/2.
Response to excess copper in the hyperthermophile Sulfolobus solfataricus strain 98/2.
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超嗜热硫磺硫化叶菌菌株 98/2 对过量铜的反应。
DOI：
10.1016/j.bbrc.2009.05.013
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发表时间：
2009
影响因子：
3.1
通讯作者：
Bini,Elisabetta
中科院分区：
文献类型：
--
作者：
Villafane,AramisA;Voskoboynik,Yekaterina;Cuebas,Mariola;Ruhl,Ilona;Bini,Elisabetta
Copper is an essential micronutrient, but toxic in excess. Sulfolobus solfataricus cells have the ability to adapt to fluctuations of copper levels in their external environment. To better understand the molecular mechanism behind the organismal response to copper, the expression of the cluster of genes copRTA, which encodes the copper-responsive transcriptional regulator CopR, the copper-binding protein CopT, and CopA, has been investigated and the whole operon has been shown to be cotranscribed at low levels from the copR promoter under all conditions, whereas increased transcription from the copTA promoter occurs in the presence of excess copper. Furthermore, the expression of the copper-transporting ATPase CopA over a 27-h interval has been monitored by quantitative real-time RT-PCR and compared to the pattern of cellular copper accumulation, as determined in a parallel analysis by Inductively Coupled Plasma Optical Emission spectrometry (ICP-OES). The results provide the basis for a model of the molecular mechanisms of copper homeostasis in Sulfolobus, which relies on copper efflux and sequestration.
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影响因子：
2.8
作者：
Ettema, Thijs J. G.;Brinkman, Arie B.;van der Oost, John
通讯作者：
van der Oost, John
影响因子：
56.9
作者：
Rae, TD;Schmidt, PJ;O'Halloran, TV
通讯作者：
O'Halloran, TV
DOI：
--
发表时间：
2008
期刊：
--
影响因子：
--
作者：
P. Blum
通讯作者：
P. Blum
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影响因子：
4.8
作者：
A. K. Mandal;Ying R. Yang;T. Kertesz;J. Argüello
通讯作者：
J. Argüello
影响因子：
13.8
作者：
Ettema, TJG;Huynen, MA;van der Oost, J
通讯作者：
van der Oost, J