Analyzing autophagosomes and mitophagosomes in the mouse brain using electron microscopy.
Analyzing autophagosomes and mitophagosomes in the mouse brain using electron microscopy.
复制标题
用电子显微镜分析小鼠脑内的自噬小体和有丝分裂噬小体。
DOI：
10.1016/j.xpro.2022.101154
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Duan K;Petralia RS;Wang YX;Li Z
电子显微镜(EM)被认为是研究宏观自噬和有丝分裂吞噬的金标准，这是大脑健康所必需的细胞过程。在这里，我们提出了一种使用EM来分析小鼠杏仁核中的自噬小体和有丝分裂噬小体的方案。我们描述了大脑切片的准备，然后是染色和EM成像。然后，我们详细介绍了识别和分析自噬体样结构和有丝分裂噬体样结构的步骤。该方法可以很容易地用于分析小鼠脑内其他区域的自噬小体和有丝分裂噬小体。有关本议定书的使用和执行的完整细节，请参阅。固定/包埋组织和切割/染色脑组织切片的电子显微镜程序在透射电子显微镜程序下获得图像，以识别/分析自噬体样和有丝分裂体样结构电子显微镜(EM)被认为是研究宏观自噬和有丝分裂吞噬的金标准，这两个过程是大脑健康的基本细胞过程。在这里，我们提出了一种使用EM来分析小鼠杏仁核中的自噬小体和有丝分裂噬小体的方案。我们描述了大脑切片的准备，然后是染色和EM成像。然后，我们详细介绍了识别和分析自噬体样结构和有丝分裂噬体样结构的步骤。该方法可以很容易地用于分析小鼠脑内其他区域的自噬小体和有丝分裂噬小体。
Electron microscopy (EM) is considered the gold standard for studying macroautophagy and mitophagy, essential cellular processes for brain health. Here, we present a protocol using EM to analyze autophagosomes and mitophagosomes in the mouse amygdala. We describe the preparation of brain sections, followed by staining and EM imaging. We then detail the steps to identify and analyze autophagosome-like and mitophagosome-like structures. This protocol can be easily adapted to analyze autophagosomes and mitophagosomes in other mouse brain regions. For complete details on the use and execution of this protocol, please refer to. Protocol to fix/embed tissue and cut/stain brain sections for electron microscopy Procedure to obtain images under the transmission electron microscope Procedure to identify/analyze autophagosome-like and mitophagosome-like structures Electron microscopy (EM) is considered the gold standard for studying macroautophagy and mitophagy, essential cellular processes for brain health. Here, we present a protocol using EM to analyze autophagosomes and mitophagosomes in the mouse amygdala. We describe the preparation of brain sections, followed by staining and EM imaging. We then detail the steps to identify and analyze autophagosome-like and mitophagosome-like structures. This protocol can be easily adapted to analyze autophagosomes and mitophagosomes in other mouse brain regions.
影响因子：
48
作者：
Schindelin, Johannes;Arganda-Carreras, Ignacio;Frise, Erwin;Kaynig, Verena;Longair, Mark;Pietzsch, Tobias;Preibisch, Stephan;Rueden, Curtis;Saalfeld, Stephan;Schmid, Benjamin;Tinevez, Jean-Yves;White, Daniel James;Hartenstein, Volker;Eliceiri, Kevin;Tomancak, Pavel;Cardona, Albert
通讯作者：
Cardona, Albert
影响因子：
16.2
作者：
Duan K;Gu Q;Petralia RS;Wang YX;Panja D;Liu X;Lehmann ML;Zhu H;Zhu J;Li Z
通讯作者：
Li Z
影响因子：
2.9
作者：
Petralia RS;Wang YX
通讯作者：
Wang YX