SlBIR3 Negatively Regulates PAMP Responses and Cell Death in Tomato.
SlBIR3 Negatively Regulates PAMP Responses and Cell Death in Tomato.
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SlBIR3 负向调节番茄中的 PAMP 反应和细胞死亡
DOI：
10.3390/ijms18091966
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发表时间：
2017-09-13
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Huang S;Nie S;Wang S;Liu J;Zhang Y;Wang X
Bri 1相关激酶1（BAK 1）相互作用受体样激酶（BIR）蛋白在模式植物拟南芥（Arabidopsis thaliana）的生长发育、病原体相关分子模式（PAMP）触发的免疫应答（PTI）和细胞死亡中起重要作用。我们在番茄（Solanum lycopersicum）中鉴定了4个BIR家族成员，包括来自A. thaliana.预测SlBIR 3编码膜定位的非精氨酸-天冬氨酸（非RD）激酶，基于蛋白质序列，其不具有自磷酸化活性，但可以在体内磷酸化。我们建立了SlBIR 3与SlBAK 1和AtBAK 1相互作用，使用酵母双杂交试验和免疫共沉淀和麦芽糖结合蛋白下拉试验。我们观察到SlBIR 3在番茄（cv. micro-tom）和A.拟南芥通过油菜素类固醇（BR）信号对生长发育的影响较弱。S1 BIR 3在A.拟南芥抑制flg 22诱导的防御反应，但不影响细菌病原体假单胞菌（PstDC 3000）的感染。使用番茄中的病毒诱导的基因沉默（VIGS）结合PstDC 3000感染证实了这一结果。在番茄（cv. micro-tom）和A.结果表明，拟南芥对灰葡萄孢的敏感性增强。此外，使用VIGS和含有SlSERK 3和SlBIR 3基因的片段的烟草脆裂病毒（TRV）-RNA 2载体使SlBIR 3与SlSERK 3A或SlSERK 3B共沉默诱导自发细胞死亡，表明在该过程中两种蛋白质之间的合作。总之，我们的研究揭示了SlBIR 3是AtBIR 3的直系同源物，并且它参与BR、PTI和细胞死亡信号通路。
Bri1-associated kinase 1 (BAK1)-interacting receptor-like kinase (BIR) proteins have been shown to play important roles in regulating growth and development, pathogen associated molecular pattern (PAMP)-triggered immunity (PTI) responses, and cell death in the model plant, Arabidopsis thaliana. We identified four BIR family members in tomato (Solanum lycopersicum), including SlBIR3, an ortholog of AtBIR3 from A. thaliana. SlBIR3 is predicted to encode a membrane localized non-arginine-aspartate (non-RD) kinase that, based on protein sequence, does not have autophosphorylation activity but that can be phosphorylated in vivo. We established that SlBIR3 interacts with SlBAK1 and AtBAK1 using yeast two-hybrid assays and co-immunoprecipitation and maltose-binding protein pull down assays. We observed that SlBIR3 overexpression in tomato (cv. micro-tom) and A. thaliana has weak effect on growth and development through brassinosteroid (BR) signaling. SlBIR3 overexpression in A. thaliana suppressed flg22-induced defense responses, but did not affect infection with the bacterial pathogen Pseudomonas syringae (PstDC3000). This result was confirmed using virus-induced gene silencing (VIGS) in tomato in conjunction with PstDC3000 infection. Overexpression of SlBIR3 in tomato (cv. micro-tom) and A. thaliana resulted in enhanced susceptibility to the necrotrophic fungus Botrytis cinerea. In addition, co-silencing SlBIR3 with SlSERK3A or SlSERK3B using VIGS and the tobacco rattle virus (TRV)-RNA2 vector containing fragments of both the SlSERK3 and SlBIR3 genes induced spontaneous cell death, indicating a cooperation between the two proteins in this process. In conclusion, our study revealed that SlBIR3 is the ortholog of AtBIR3 and that it participates in BR, PTI, and cell death signaling pathways.
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通过蛋白质糖基化对拟南芥BAK1/SERK4调节的细胞死亡的特定控制。
影响因子：
18
作者：
de Oliveira MV;Xu G;Li B;de Souza Vespoli L;Meng X;Chen X;Yu X;de Souza SA;Intorne AC;de A Manhães AM;Musinsky AL;Koiwa H;de Souza Filho GA;Shan L;He P
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He P
影响因子：
11.6
作者：
Albrecht, C;Russinova, E;de Vries, S
通讯作者：
de Vries, S
影响因子：
64.8
作者：
Chinchilla, Delphine;Zipfel, Cyril;Boller, Thomas
通讯作者：
Boller, Thomas
影响因子：
30.3
作者：
Gao, Minghui;Wang, Xia;Zhang, Yuelin
通讯作者：
Zhang, Yuelin
影响因子：
16
作者：
Kim TW;Guan S;Burlingame AL;Wang ZY
通讯作者：
Wang ZY