Adropin preserves the blood-brain barrier through a Notch1/Hes1 pathway after intracerebral hemorrhage in mice.
Adropin preserves the blood-brain barrier through a Notch1/Hes1 pathway after intracerebral hemorrhage in mice.
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Adropin 通过 Notch1/Hes1 通路保护小鼠脑出血后的血脑屏障
DOI：
10.1111/jnc.14238
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发表时间：
2017-12
影响因子：
4.7
通讯作者：
Zhang JH
中科院分区：
文献类型：
--
作者：
Yu L;Lu Z;Burchell S;Nowrangi D;Manaenko A;Li X;Xu Y;Xu N;Tang J;Dai H;Zhang JH
Adropin在中枢神经系统（CNS）中表达，在卒中的发生中起着至关重要的作用。然而，目前很少有人知道肾上腺素对脑出血（ICH）后血脑屏障（BBB）功能的影响。在这项研究中，在胶原酶诱导的脑出血小鼠中的作用进行了研究。在ICH后1小时，通过鼻内给予小鼠重组人阿霉素。在不同时间间隔测量脑含水量、血脑屏障通透性和神经功能。使用Western印迹分析定量蛋白质，并通过免疫荧光染色观察Adropin和Notch 1的定位。结果表明，Adropin降低了脑含水量，改善了神经功能。Adropin通过增加N-钙粘蛋白表达和减少白蛋白外渗来保护BBB的功能。此外，在体内敲低Notch 1和Hes 1都取消了adropin的保护作用。综上所述，我们的数据表明，Adropin通过保护BBB和通过Notch 1信号通路改善功能结局，构成了ICH的潜在治疗价值。Adropin在中枢神经系统（CNS）中表达，在卒中的发生中起着至关重要的作用。在这项研究中，在胶原酶诱导的脑出血小鼠中的作用进行了研究。我们的研究表明，Adropin通过Notch 1信号通路保护BBB和改善功能结局，构成了ICH的潜在治疗价值。
Adropin is expressed in the central nervous system (CNS) and plays a crucial role in the development of stroke. However, little is currently known about the effects of adropin on the blood-brain barrier (BBB) function after intracerebral hemorrhage (ICH). In this study, the role of adropin in collagenase-induced ICH was investigated in mice. At 1-h post ICH, mice were administered with recombinant human adropin by intranasal. Brain water content, BBB permeability, and neurological function were measured at different time intervals. Proteins were quantified using Western blot analysis, and the localizations of adropin and Notch1 were visualized via immunofluorescence staining. It is shown that adropin reduced brain water content and improved neurological functions. Adropin preserved the functionality of BBB by increasing N-cadherin expression and reducing extravasation of albumin. Moreover, in vivo knockdown of Notch1 and Hes1 both abolished the protective effects of adropin. Taken together, our data demonstrate that adropin constitutes a potential treatment value for ICH by preserving BBB and improving functional outcomes through the Notch1 signaling pathway. Adropin is expressed in the central nervous system (CNS) and plays a crucial role in the development of stroke. In this study, the role of adropin in collagenase-induced ICH was investigated in mice. Our research demonstrates that adropin constitutes a potential treatment value for ICH by preserving BBB and improving functional outcomes through the Notch1 signaling pathway.
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影响因子：
29
作者：
Kumar KG;Trevaskis JL;Lam DD;Sutton GM;Koza RA;Chouljenko VN;Kousoulas KG;Rogers PM;Kesterson RA;Thearle M;Ferrante AW Jr;Mynatt RL;Burris TP;Dong JZ;Halem HA;Culler MD;Heisler LK;Stephens JM;Butler AA
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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