Lateral hypothalamic neuronal ensembles regulate pre-sleep nest-building behavior.
Lateral hypothalamic neuronal ensembles regulate pre-sleep nest-building behavior.
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DOI：
10.1016/j.cub.2021.12.053
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发表时间：
2022-02-28
期刊：
影响因子：
9.2
通讯作者：
Eban-Rothschild, Ada
中科院分区：
文献类型：
--
作者：
Sotelo, Maria, I;Tyan, Jean;Markunas, Chelsea;Sulaman, Bibi A.;Horwitz, Lorraine;Lee, Hankyu;Morrow, Joshua G.;Rothschild, Gideon;Duan, Bo;Eban-Rothschild, Ada
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The transition from wakefulness to sleep requires striking alterations in brain activity, physiology and behavior, yet the precise neuronal circuit elements facilitating this transition remain unclear. Prior to sleep onset, many animal species display characteristic behaviors, including finding a safe location, performing hygiene-related behaviors and preparing a space for sleep. It has been proposed that the pre-sleep period is a transitional phase in which engaging in a specific behavioral repertoire de-arouses the brain and facilitates the wake-to-sleep transition, yet both causal evidence for this premise and an understanding of the neuronal circuit elements involved are lacking. Here, we combine detailed behavioral observations, EEG-EMG recordings and selective targeting and activity modulation of pre-sleep-active neurons to reveal the behaviors preceding sleep initiation and their underlying neurobiological mechanisms. We show that mice engage in temporally structured behaviors with stereotypic EEG signatures prior to sleep and that nest-building and grooming become significantly more prevalent with sleep proximity. We next demonstrate that the ability to build a nest promotes the initiation and consolidation of sleep and that the lack of nesting material chronically fragments sleep. Lastly, we identify broadly projecting and predominantly glutamatergic neuronal ensembles in the lateral hypothalamus that regulate the motivation to engage in pre-sleep nest-building behavior and gate sleep initiation and intensity. Our study provides causal evidence for the facilitatory role of pre-sleep behaviors in sleep initiation and consolidation, as well as a functional characterization of the neuronal underpinnings regulating a sleep-related and goal-directed complex behavior. Sotelo et al. demonstrate that mice have a temporally structured pre-sleep phase dominated by nesting and grooming. They show that the capacity to build and sleep in a nest promotes sleep initiation and consolidation and that pre-sleep nesting is controlled by non-hypocretin glutamatergic LH ensembles that also gate sleep initiation and intensity.
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影响因子：
64.5
作者：
Duan B;Cheng L;Bourane S;Britz O;Padilla C;Garcia-Campmany L;Krashes M;Knowlton W;Velasquez T;Ren X;Ross S;Lowell BB;Wang Y;Goulding M;Ma Q
通讯作者：
Ma Q
影响因子：
16.6
作者：
González JA;Iordanidou P;Strom M;Adamantidis A;Burdakov D
通讯作者：
Burdakov D
影响因子：
25
作者：
Eban-Rothschild A;Rothschild G;Giardino WJ;Jones JR;de Lecea L
通讯作者：
de Lecea L
影响因子：
25
作者：
DeNardo, Laura A.;Liu, Cindy D.;Luo, Liqun
通讯作者：
Luo, Liqun
影响因子：
2.8
作者：
Eban-Rothschild, Ada D.;Bloch, Guy
通讯作者：
Bloch, Guy