Discrete cis-active genomic sequences dictate the pituitary cell type-specific expression of rat prolactin and growth hormone genes
Discrete cis-active genomic sequences dictate the pituitary cell type-specific expression of rat prolactin and growth hormone genes
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离散顺式活性基因组序列决定大鼠催乳素和生长激素基因的垂体细胞类型特异性表达
DOI：
10.1038/322557a0
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发表时间：
1986
期刊：
影响因子：
64.8
通讯作者：
Michael G. Rosenfeld
中科院分区：
文献类型：
--
作者：
C. Nelson;E. Crenshaw;R. Franco;S. Lira;V. Albert;Ronald M. Evans;Ronald M. Evans;Michael G. Rosenfeld;Michael G. Rosenfeld
垂体前叶源于起源于Rathke眼袋的共同原基，包含表型不同的细胞类型，每种细胞类型表达不同的营养激素：促肾上腺皮质激素（ACTH）、促甲状腺激素（TSH）、催乳素、生长激素和促卵泡激素(FSH)/促黄体生成素（LH）（参考文献1）。结构上相关的催乳素和生长激素基因，进化上来源于单一的原始基因2，分别在不同的细胞类型中表达——乳营养细胞和生长营养细胞——它们的表达实际上仅限于垂体1。垂体激素表现出发育表达的时间模式，大鼠生长激素和催乳素通常是最后表达的激素3 - 8。据报道，这两种结构相关的神经内分泌基因在成熟乳营养细胞出现之前的单个细胞内、成熟垂体前叶细胞亚群和许多垂体腺瘤中共同表达，这提出了催乳素和生长激素基因在发育过程中受共同因素控制的可能性。我们现在分别报道了大鼠催乳素和生长激素基因5 '侧区域核苷酸序列的鉴定和表征，它们以位置和取向无关的方式将细胞特异性表达转移到异源基因中。生长激素基因组序列发挥其作用所需的至少一个假定的反式作用因子显然不同于调节催乳素基因的相应增强元件的因子，因为产生催乳素而不产生生长激素的垂体“乳营养”细胞系选择性地不能表达含有生长激素增强序列的融合基因。
The anterior pituitary gland, which is derived from a common primordium originating in Rathke's pouch, contains phenotypically distinct cell types, each of which express discrete trophic hormones: adrenocorticotropic hormone (ACTH), thyroid-stimulating hormone (TSH), prolactin, growth hormone, and follicle stimulating hormone (FSH)/luteinizing hormone (LH) (reviewed in ref. 1). The structurally related prolactin and growth hormone genes, which are evolutionarily derived from a single primordial gene2, are expressed in discrete cell types—lactotrophs and somatotrophs, respectively—with their expression virtually limited to the pituitary gland1. The pituitary hormones exhibit a temporal pattern of developmental expression with rat growth hormone and prolactin characteristically being the last hormones expressed3–8. The reported co-expression of these two structurally related neuroendocrine genes within single cells prior to the appearance of mature lactotrophs, in a subpopulation of mature anterior pituitary cells, and in many pituitary adenomas1,6–9 raises the possibility that the prolactin and growth hormone genes are developmentally controlled by a common factor(s). We now report the identification and characterization of nucleotide sequences in the 5′-flanking regions of the rat prolactin and growth hormone genes, respectively, which act in a position- and orientation-independent fashion to transfer cell-specific expression to heterologous genes. At least one putative trans-acting factor required for the growth hormone genomic sequence to exert its effects is apparently different from those modulating the corresponding enhancer element(s) of the prolactin gene because a pituitary ‘lactotroph’ cell line producing prolactin but not growth hormone selectively fails to express fusion genes containing the growth hormone enhancer sequence.
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下丘脑对大鼠围产期催乳素分泌的控制。
影响因子：
4.8
作者：
Khorram,O;Depalatis,LR;McCann,SM
通讯作者：
McCann,SM
大鼠垂体肿瘤细胞的新克隆株：催乳素非调节分泌的模型。
DOI：
10.1530/acta.0.1060459
发表时间：
1984
期刊：
Acta endocrinologica
影响因子：
--
作者：
Reymond,MJ;Nansel,DD;Burrows,GH;Neaves,WB;Porter,JC
通讯作者：
Porter,JC
雌二醇受体与大鼠催乳素基因上游至少一千碱基的区域选择性结合。
DOI：
10.1089/dna.1985.4.1
发表时间：
1985
期刊：
DNA (Mary Ann Liebert, Inc.)
影响因子：
--
作者：
Maurer,RA
通讯作者：
Maurer,RA
Fischer 344 大鼠垂体肿瘤中催乳素基因域的染色质结构、转录和甲基化。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Durrin,LK;Weber,JL;Gorski,J
通讯作者：
Gorski,J