Lipid hydroperoxide stimulates leukocyte-endothelium interaction in the retinal microcirculation.
Lipid hydroperoxide stimulates leukocyte-endothelium interaction in the retinal microcirculation.
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脂质过氧化氢刺激视网膜微循环中的白细胞-内皮相互作用。
DOI：
10.1006/exer.2002.1178
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发表时间：
2002
影响因子：
3.4
通讯作者：
Y. Ogura
中科院分区：
文献类型：
--
作者：
K. Tamai;A. Matsubara;K. Tomida;Y. Matsuda;Hiroshi Morita;D. Armstrong;Y. Ogura
将不同量（1，5，10或100 μ g）的LHP（18：2）溶于5 μ l的硼酸钠缓冲液（SBB，0.02M）注入Brown-Norway大鼠玻璃体，观察玻璃体内脂质过氧化氢（LHP）暴露后视网膜微循环中白细胞的动态变化。作为对比研究，以相同的方式注射溶解在5 μ l SBB中的10 μ g亚油酸（LA）。将未接受注射的大鼠评价为未给药。在LHP暴露后2至48小时，检查以下内容：（1）沿视网膜大静脉沿着滚动的白细胞的流量，（2）使用吖啶橙子数字荧光照相术在视网膜微血管中积累的白细胞的数量，以及（3）视网膜大血管的直径。在LHP处理的眼睛中，观察到白细胞沿着视网膜大静脉沿着，并且数量以剂量依赖性方式增加（1至10 μ g）。LHP（10-100 μ g）注射后6小时，滚动的白细胞流量达到峰值。在LA治疗或未治疗的眼睛中未观察到滚动的白细胞。LHP（10 μ g）注射后4小时，累积的白细胞数量开始增加，24小时达到峰值。该数字显着高于LA治疗和未治疗的眼睛。注射LHP（10 μ g）后4小时开始观察到静脉扩张，与LA治疗和未治疗的眼睛相比，在6小时和24小时变得显著。结果表明，由于氧化应激导致的玻璃体中LHP水平的增加增强了视网膜微循环中白细胞-内皮素的相互作用。
Leukocyte dynamics were evaluatyed in vivo in rat retinal microcirculation following exposure to lipid hydroperoxide (LHP) in the vitreous.Various amounts (1, 5, 10 or 100 microg) of LHP (18:2) dissolved in 5 microl of sodium borate buffer (SBB, 0.02M) were injected into the vitreous of Brown-Norway rats. As a comparative study, 10 microg of linoleic acid (LA) dissolved in 5 microl of SBB was injected in the same way. Rats that did not undergo injection were evaluated as un-treated. At 2 to 48 hr after LHP exposure, the following were examined: (1) the flux of rolling leukocytes along the major retinal veins, (2) the number of leukocytes that accumulated in the retinal microvasculature using acridine orange digital fluorography and (3) the diameter of major retinal vessels. In the LHP-treated eyes, leukocyte rolling along the major retinal veins was observed and the number increased in a dose-dependent manner ( 1 to 10 microg). The flux of rolling leukocytes peaked at 6 hr after LHP (10-100 microg) injection. No rolling leukocytes were observed in LA-treated or un-treated eyes. The number of accumulated leukocytes started to increase at 4 hr and peaked at 24 hr after LHP (10 microg) injection. This number was significantly higher than that in LA-treated and un-treated eyes. Venous dilation was seen from 4 hr after LHP (10 microg) injection and became significant at 6 and 24 hr as compared with LA-treated and un-treated eyes. The results indicate that increased LHP levels in the vitreous due to oxidative stress enhance leukocyte-enothelium interaction in the retinal microcirculation.
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影响因子：
6.5
作者：
A. Girotti
通讯作者：
A. Girotti
影响因子：
6.1
作者：
BHUYAN, KC;BHUYAN, DK;PODOS, SM
通讯作者：
PODOS, SM
影响因子：
7.4
作者：
Yang, H;Shi, MJ;Guo, ZM
通讯作者：
Guo, ZM
反应性氧化剂介导 TNF-α 诱导的白细胞与大鼠肠系膜小静脉内皮的粘附。
DOI：
10.1152/ajpheart.1995.269.6.h1833
发表时间：
1995
期刊：
The American journal of physiology
影响因子：
--
作者：
Morita,Y;Clemens,MG;Miller,LS;Rangan,U;Kondo,S;Miyasaka,M;Yoshikawa,T;Bulkley,GB
通讯作者：
Bulkley,GB
影响因子：
7.7
作者：
Gunnett, CA;Heistad, DD;Faraci, FM
通讯作者：
Faraci, FM