Intraglomerular basement membrane translocation of immune complex (IC) in the development of passive in situ IC nephritis of rats.
Intraglomerular basement membrane translocation of immune complex (IC) in the development of passive in situ IC nephritis of rats.
复制标题
免疫复合物（IC）的肾小球内基底膜易位在大鼠被动原位IC肾炎的发展中。
作者：
Y. Fujigaki;M. Nagase;N. Honda
本研究旨在阐明荷基免疫复合体肾炎的发病机制。用免疫电子显微镜观察肾炎形成后的时间变化，以明确抗原(Ag)在通过肾小球基底膜(GBM)移位过程中是否与抗体(Ab)和补体C3结合。肾内注入阳离子铁蛋白(PI>；10.0)作为抗原，15分钟后注射兔抗铁蛋白抗体，观察到内皮下Ag-Ab-C3复合体的沉积。2小时至1天，致密层内可见大量与抗体密切结合的抗原，但仅检测到少量C3。在此期间，内皮下区的Ag和Ab逐渐减少。但1d后C3与Ag-Ab复合体一起重新出现在上皮下区，内皮下C3明显减少。在浸泡固定后2小时和第1天，无论用磷酸盐缓冲液灌流，肾小球基底膜内Ag和Ab的分布相似。另一方面，与对照组相比，实验组大鼠通过致密层的时间延迟。第1天也出现明显的蛋白尿。尽管普遍认为抗体与阳离子Ag结合会导致低亲和力的免疫复合体，但阳离子Ag与抗体密切相关地跨基底膜转移。补体在内皮下和上皮下间隙中被双相激活。内皮下补体的激活可能参与了免疫复合体的移位。
A study was performed to elucidate the mechanisms of charge-based immune complex nephritis. A chronological observation after induction of nephritis was made by immunoelectron microscopy to clarify whether antigen (Ag) remains in association with antibody (Ab) and C3 during the translocation through the glomerular basement membrane (GBM). Fifteen minutes after intrarenal perfusion with cationized ferritin (pI > 10.0) as Ag, followed by injection of rabbit anti-ferritin Ab, deposition of subendothelial Ag-Ab-C3 complexes was observed. Between 2 hours and 1 day, a large number of Ag in close association with Ab was noted in the lamina densa, but only a small amount of C3 was detectable. During this time Ag and Ab in the subendothelial region gradually decreased. However, C3 reappeared in the subepithelial region together with the Ag-Ab complex after 1 day, and the subendothelial C3 significantly decreased. At 2 hours and day 1, the distributions of Ag and Ab in the GBM were similar in immersion-fixed kidneys regardless of the preperfusion with phosphate-buffered saline. On the other hand, the passage of Ag across the lamina densa was delayed in the experimental rats as compared with the controls. Significant albuminuria also appeared on day 1. Despite the general concept that Ab binding to cationized Ag results in low avidity immune complex, cationized Ag translocated across the GBM in close association with Ab. The complement was activated biphasically in the subendothelial and in the subepithelial space. The subendothelial complement activation may have contributed to the translocation of immune complex.
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影响因子：
19.6
作者：
Makino,H;Lelongt,B;Kanwar,YS
通讯作者：
Kanwar,YS
C6 消耗可减少非肾小球抗原诱导的实验性肾病中的蛋白尿。
DOI：
10.1681/asn.v24894
发表时间：
1991
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
Couser,WG;Ochi,RF;Baker,PJ;Schulze,M;Campbell,C;Johnson,RJ
通讯作者：
Johnson,RJ
影响因子：
15.9
作者：
SALANT, DJ;BELOK, S;COUSER, WG
通讯作者：
COUSER, WG
抗体诱导的海曼抗原在培养的肾小球内脏上皮细胞表面的重新分布：在海曼肾小球肾炎的发病机制中可能发挥作用。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Camussi,G;Brentjens,JR;Noble,B;Kerjaschki,D;Malavasi,F;Roholt,OA;Farquhar,MG;Andres,G
通讯作者：
Andres,G
免疫沉积物的形成和疾病。
DOI：
--
发表时间：
1986
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Andres,G;Brentjens,JR;Caldwell,PR;Camussi,G;Matsuo,S
通讯作者：
Matsuo,S