Preferential secretion of collagen type 3 versus type 1 from adventitial fibroblasts stimulated by TGF-β/Smad3-treated medial smooth muscle cells.
Preferential secretion of collagen type 3 versus type 1 from adventitial fibroblasts stimulated by TGF-β/Smad3-treated medial smooth muscle cells.
复制标题
DOI：
10.1016/j.cellsig.2012.12.021
复制
发表时间：
2013-04
影响因子：
4.8
通讯作者：
Kent KC
中科院分区：
文献类型：
--
作者：
Goel SA;Guo LW;Shi XD;Kundi R;Sovinski G;Seedial S;Liu B;Kent KC
在接受血管成形术的患者中，30 - 50%发生再狭窄或动脉管腔再狭窄。适应性重塑是血管尺寸的代偿性增大，并且已经报道可以防止再狭窄的有害影响。我们的前期研究表明，中膜层转化生长因子β（transforming growth factor，TGF-β）及其信号蛋白Smad3的升高可诱导血管成形术后大鼠颈动脉适应性重构，并伴有外膜总胶原的增加。为了深入了解胶原蛋白在Smad3诱导的适应性重塑中可能发挥的作用，我们研究了中膜平滑肌细胞（SMCs）和外膜成纤维细胞在调节两种主要胶原蛋白亚型分泌中的细胞间通讯机制。我们已经确定了在TGF-β 1处理/Smad3表达的中膜平滑肌细胞（SMC）的条件培养基刺激后，外膜成纤维细胞优先分泌胶原蛋白-3而不是胶原蛋白-1，与对照培养基相比，SMC含有更高水平的CTGF和IGF 2。用针对CTGF或IGF2的siRNA处理TGF-β/Smad3刺激的SMC逆转了条件培养基对成纤维细胞优先分泌胶原蛋白-3的作用。此外，重组CTGF和IGF2一起刺激外膜成纤维细胞优先分泌胶原-3而不是胶原-1。这是第一项鉴定由于TGF-β/Smad3刺激中膜SMC而从外膜成纤维细胞优先分泌胶原蛋白-3相对于胶原蛋白-1的研究，并且CTGF和IGF 2一起起作用以介导两种细胞类型之间的这种信号传导。
Restenosis, or arterial lumen re-narrowing, occurs in 30–50% of the patients undergoing angioplasty. Adaptive remodeling is the compensatory enlargement of the vessel size, and has been reported to prevent the deleterious effects of restenosis. Our previous studies have shown that elevated transforming growth factor (TGF-β) and its signaling protein Smad3 in the media layer induce adaptive remodeling of angioplastied rat carotid artery accompanying an increase of total collagen in the adventitia. In order to gain insights into a possible role of collagen in Smad3-induced adaptive remodeling, here we have investigated a mechanism of cell–cell communication between medial smooth muscle cells (SMCs) and adventitial fibroblasts in regulating the secretion of two major collagen subtypes. We have identified a preferential collagen-3 versus collagen-1 secretion by adventitial fibroblasts following stimulation by the conditioned medium from the TGF-β1-treated/Smad3-expressing medial smooth muscle cells (SMCs), which contained higher levels of CTGF and IGF2 as compared to control medium. Treating the TGF-β/ Smad3-stimulated SMCs with an siRNA to either CTGF or IGF2 reversed the effect of conditioned media on preferential collagen-3 secretion from fibroblasts. Moreover, recombinant CTGF and IGF2 together stimulated adventitial fibroblasts to preferentially secrete collagen-3 versus collagen-1. This is the first study to identify a preferential secretion of collagen-3 versus collagen-1 from adventitial fibroblasts as a result of TGF-β/Smad3 stimulation of medial SMCs, and that CTGF and IGF2 function together to mediate this signaling communication between the two cell types.
登录
查看更多内容
影响因子：
37.8
作者：
Qiu, Hongyu;Depre, Christophe;Vatner, Stephen F.
通讯作者：
Vatner, Stephen F.
DOI：
10.1016/j.jss.2010.12.020
发表时间：
2011-05-15
期刊：
The Journal of surgical research
影响因子：
--
作者：
Suwanabol PA;Kent KC;Liu B
通讯作者：
Liu B
影响因子：
4.6
作者：
Qiu, Hongyu;Tian, Bin;Depre, Christophe
通讯作者：
Depre, Christophe
影响因子：
10.8
作者：
Kundi, Rishi;Hollenbeck, Scott T.;Kent, K. Craig
通讯作者：
Kent, K. Craig
影响因子：
7.7
作者：
Lam, S;van der Geest, RN;van Kooten, C
通讯作者：
van Kooten, C