The Rad9-Rad1-Hus1 DNA Repair Clamp is Found in Microsporidia.
The Rad9-Rad1-Hus1 DNA Repair Clamp is Found in Microsporidia.
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DOI：
10.1093/gbe/evac053
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发表时间：
2022-04-10
影响因子：
3.3
通讯作者：
Pombert, Jean-Francois
中科院分区：
文献类型：
--
作者：
Dos Santos, Anne Caroline Mascarenhas;Julian, Alexander Thomas;Pombert, Jean-Francois
关键词：
DNA修复是基因组完整性的重要组成部分，修复能力降低的生物体往往会以较高的速率积累突变。微孢子虫是一种细胞内寄生虫，表现出高水平的遗传分化，据推测源于几种蛋白质的缺乏，包括异源三聚体Rad9-Rad1-Hus1 DNA修复钳。来自脑虫科的微孢子虫物种经历了严重的流线型，其小基因组编码约2000种蛋白质。在微孢子虫中发现的高度分化的序列使得功能推断变得困难，以至于这2000种蛋白质中大约有一半没有已知的功能。利用结合蛋白结构预测和三维相似性搜索的基于结构同源的注释方法，我们发现Rad9-Rad1-Hus1 DNA夹存在于微孢子虫中，以及以前认为在这些生物体中缺失的许多其他DNA修复机制组件。总之，我们的结果表明，DNA修复机制是存在的，并且可能在微孢子虫中起作用。
DNA repair is an important component of genome integrity and organisms with reduced repair capabilities tend to accumulate mutations at elevated rates. Microsporidia are intracellular parasites exhibiting high levels of genetic divergence postulated to originate from the lack of several proteins, including the heterotrimeric Rad9–Rad1–Hus1 DNA repair clamp. Microsporidian species from the Encephalitozoonidae have undergone severe streamlining with small genomes coding for about 2,000 proteins. The highly divergent sequences found in Microsporidia render functional inferences difficult such that roughly half of these 2,000 proteins have no known function. Using a structural homology-based annotation approach combining protein structure prediction and tridimensional similarity searches, we found that the Rad9–Rad1–Hus1 DNA clamp is present in Microsporidia, together with many other components of the DNA repair machinery previously thought to be missing from these organisms. Altogether, our results indicate that the DNA repair machinery is present and likely functional in Microsporidia.
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影响因子：
16.6
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影响因子：
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作者：
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通讯作者：
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