Evolutionary Genomics of Metchnikovella incurvata (Metchnikovellidae): An Early Branching Microsporidium.
Evolutionary Genomics of Metchnikovella incurvata (Metchnikovellidae): An Early Branching Microsporidium.
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DOI：
10.1093/gbe/evy205
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发表时间：
2018-10-01
影响因子：
3.3
通讯作者：
López-García P
中科院分区：
文献类型：
--
作者：
Galindo LJ;Torruella G;Moreira D;Timpano H;Paskerova G;Smirnov A;Nassonova E;López-García P
微寄生虫是一种高度特化的超寄生虫，它感染并在栖息在海洋无脊椎动物体内的gregarines（Apicomplexa）内繁殖。它们的系统发育关系一直在讨论之中，直到最近，当对第一个接近完整的metchavellid基因组，即Amphiamblys sp.，将其置于相对于大多数微孢子虫的基部位置。微孢子虫是一个高度多样化的谱系，与后孔门的全菌门分支内的Rozellida（Rozellosporidia = Rozellomycota = Cryptomycota）有关。通过对单个分离的感染gregarine细胞的DNA进行测序，我们获得了第二种metchnikovella vellid物种的几乎完整的基因组，以及第一种分类学描述和记录良好的物种Metchnikovella invervata。我们的基因组分析表明，尽管M. incurvata与Amphiamplys sp.形成一个单系群，证实了微孢子虫是微孢子虫的深层分支之一。比较基因组分析表明，与大多数微孢子虫一样，微孢子虫缺乏参与能量转导的线粒体基因，因此不能通过线粒体氧化磷酸化合成自己的ATP。它们也缺乏在大多数微孢子虫中广泛存在的水平获得的ATP转运蛋白。我们推测，一个家庭的线粒体载体蛋白进化到运输ATP从主机到metchalvellid细胞。我们观察到，在微孢子虫的进化路径中，参与DNA修复途径的基因沿着逐渐减少，这可能至少部分地解释了大多数衍生物种的极高进化率。我们的数据还表明，基因组减少和收购的新基因共同发生在微孢子虫适应他们的主机。
Metchnikovellids are highly specialized hyperparasites, which infect and reproduce inside gregarines (Apicomplexa) inhabiting marine invertebrates. Their phylogenetic affiliation was under constant discussion until recently, when analysis of the first near-complete metchnikovellid genome, that of Amphiamblys sp., placed it in a basal position with respect to most Microsporidia. Microsporidia are a highly diversified lineage of extremely reduced parasites related to Rozellida (Rozellosporidia = Rozellomycota = Cryptomycota) within the Holomycota clade of Opisthokonta. By sequencing DNA from a single-isolated infected gregarine cell we obtained an almost complete genome of a second metchnikovellid species, and the first one of a taxonomically described and well-documented species, Metchnikovella incurvata. Our phylogenomic analyses show that, despite being considerably divergent from each other, M. incurvata forms a monophyletic group with Amphiamplys sp., and confirm that metchnikovellids are one of the deep branches of Microsporidia. Comparative genomic analysis demonstrates that, like most Microsporidia, metchnikovellids lack mitochondrial genes involved in energy transduction and are thus incapable of synthesizing their own ATP via mitochondrial oxidative phosphorylation. They also lack the horizontally acquired ATP transporters widespread in most Microsporidia. We hypothesize that a family of mitochondrial carrier proteins evolved to transport ATP from the host into the metchnikovellid cell. We observe the progressive reduction of genes involved in DNA repair pathways along the evolutionary path of Microsporidia, which might explain, at least partly, the extremely high evolutionary rate of the most derived species. Our data also suggest that genome reduction and acquisition of novel genes co-occurred during the adaptation of Microsporidia to their hosts.
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影响因子：
16.6
作者：
Dean P;Sendra KM;Williams TA;Watson AK;Major P;Nakjang S;Kozhevnikova E;Goldberg AV;Kunji ERS;Hirt RP;Embley TM
通讯作者：
Embley TM
影响因子：
16.6
作者：
Boniecki MT;Freibert SA;Mühlenhoff U;Lill R;Cygler M
通讯作者：
Cygler M
来自微孢子虫脑肠道的最小已知核基因组的完整序列。
影响因子：
16.6
作者：
Corradi, Nicolas;Pombert, Jean-Francois;Farinelli, Laurent;Didier, Elizabeth S.;Keeling, Patrick J.
通讯作者：
Keeling, Patrick J.
DOI：
10.1111/jeu.12519
发表时间：
2018-11
期刊：
The Journal of eukaryotic microbiology
影响因子：
--
作者：
Bass D;Czech L;Williams BAP;Berney C;Dunthorn M;Mahé F;Torruella G;Stentiford GD;Williams TA
通讯作者：
Williams TA
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T