Gene length as a regulator for ribosome recruitment and protein synthesis: theoretical insights.
Gene length as a regulator for ribosome recruitment and protein synthesis: theoretical insights.
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DOI：
10.1038/s41598-017-17618-1
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发表时间：
2017-12-12
影响因子：
4.6
通讯作者：
Ciandrini L
中科院分区：
文献类型：
--
作者：
Fernandes LD;Moura APS;Ciandrini L
在翻译水平上，蛋白质合成速率是由转录物序列的特性决定的。mRNA的效率可以通过改变控制核糖体招募的核糖体结合位点或确定蛋白质延伸速度的密码子使用来调节。在这项工作中，我们提出转录长度是翻译效率的另一个关键决定因素。基于一个物理模型，考虑核糖体在mRNA上前进并在其周围扩散的动力学，以及mRNA循环和核糖体脱落，我们解释了转录物长度如何在建立核糖体招募和mRNA的整体翻译速率中发挥核心作用。根据我们的研究结果，3 '端靠近mRNA的核糖体招募位点可以在翻译过程中诱导有利于核糖体再循环的反馈。我们还演示了这个过程如何参与形成实验性核糖体密度-基因长度依赖。最后，我们认为细胞可以利用这种机制来调节和平衡其核糖体资源的使用。
Protein synthesis rates are determined, at the translational level, by properties of the transcript’s sequence. The efficiency of an mRNA can be tuned by varying the ribosome binding sites controlling the recruitment of the ribosomes, or the codon usage establishing the speed of protein elongation. In this work we propose transcript length as a further key determinant of translation efficiency. Based on a physical model that considers the kinetics of ribosomes advancing on the mRNA and diffusing in its surrounding, as well as mRNA circularisation and ribosome drop-off, we explain how the transcript length may play a central role in establishing ribosome recruitment and the overall translation rate of an mRNA. According to our results, the proximity of the 3′ end to the ribosomal recruitment site of the mRNA could induce a feedback in the translation process that would favour the recycling of ribosomes. We also demonstrate how this process may be involved in shaping the experimental ribosome density-gene length dependence. Finally, we argue that cells could exploit this mechanism to adjust and balance the usage of its ribosomal resources.
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影响因子：
5.4
作者：
Li GW
通讯作者：
Li GW
影响因子：
3.4
作者：
Chou, T
通讯作者：
Chou, T
mRNA翻译中的供求动力。
影响因子：
4.3
作者：
Brackley CA;Romano MC;Thiel M
通讯作者：
Thiel M
长度依赖性翻译起始有利于人类细胞的功能蛋白质组。
影响因子：
--
作者：
Guo, Jieming;Lian, Xinlei;Zhang, Gong
通讯作者：
Zhang, Gong
影响因子：
4.3
作者：
Ciandrini L;Stansfield I;Romano MC
通讯作者：
Romano MC