Morphine and norepinephrine but not 5-hydroxytryptamine and γ-aminobutyric acid inhibit the potassium-stimulated release of substance P from rat spinal cord slices
Morphine and norepinephrine but not 5-hydroxytryptamine and γ-aminobutyric acid inhibit the potassium-stimulated release of substance P from rat spinal cord slices
复制标题
吗啡和去甲肾上腺素抑制大鼠脊髓切片中 P 物质的钾刺激释放，但 5-羟色胺和 γ-氨基丁酸不抑制
DOI：
10.1016/0006-8993(86)90189-7
复制
发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
M. Vasko
中科院分区：
文献类型：
--
作者：
I. Pang;M. Vasko
本文研究了吗啡、去甲肾上腺素（NE）、5-羟色胺（5-HT）和γ-氨基丁酸（GABA）对钾刺激的大鼠脊髓片P物质（SP）释放的抑制作用。将雄性Sprague-Dawley大鼠断头，取出一段2 cm腰骶脊髓，切成0.5 × 0.5 mm的碎片，称重，置于灌注室中，并在37 ℃下用改良的Krebs碳酸氢盐缓冲液灌注。收集Perf USAte，冻干，然后使用放射免疫测定法测定SP。将脊髓组织暴露于50 mM KCl中8 min，可使SP的释放产生钙依赖性增加，从约0.1 pg/mg组织/min的基础水平增加至0.3 pg/mg组织/min。浓度为10− 4和10− 5 M的吗啡和NE并不改变基础水平，但可显著减少钾刺激的SP释放。纳洛酮（10− 5 M）和酚妥拉明（10 - 5 M）不影响SP的释放，但分别减弱吗啡和NE的作用。纳洛酮不拮抗NE的释放抑制作用，酚妥拉明也不阻断吗啡的作用，提示两种激动剂的作用是独立的。相反，浓度为10− 4 M和10− 4 M的5-HT和GABA并没有显著改变基础或钾刺激的SP释放。这些结果表明，脊髓中SP释放的差异调节，并支持吗啡和NE可能部分通过抑制脊髓中SP的释放来改变伤害性感受的假设。
We studied whether morphine, norepinephrine (NE), 5-hydroxytryptamine (5-HT) and γ-aminobutyric acid (GABA) inhibit the potassium-stimulated release of substance P (SP) from rat spinal cord slices. Male Sprague-Dawley rats were decapitated and a 2-cm segment of lumbosacral spinal cord was removed, chopped into 0.5 × 0.5 mm pieces, weighed, placed in a perfusion chamber and perfused at 37°C with a modified Krebs bicarbonate buffer. Perf USAte was collected, lyophilized, then assayed for SP using radioimmunoassay. Exposure of spinal cord tissue to 50 mM KCl for 8 min produced a calcium-dependent increase in the release of SP from a basal level of approximately 0.1 pg/mg tissue/min to 0.3 pg/mg tissue/min. Morphine and NE at concentrations of 10−4and 10−5M did not alter basal release but caused a significant reduction in the potassium-stimulated release of SP. Naloxone (10−5M) and phentolamine (10−5M) did not affect SP release but attenuated the effects of morphine and NE, respectively. Naloxone did not antagonize the inhibition of release produced by NE nor did phentolamine block the effect of morphine, suggesting that the actions of the agonists are independent. In contrast, 5-HT and GABA at concentrations of 10−4M and 10−4M did not significantly alter the basal or potassium-stimulated release of SP. These results demonstrate a differential regulation of SP release in the spinal cord and support the hypothesis that morphine and NE may modify nociception, in part, by inhibiting the release of SP in the spinal cord.
去甲肾上腺素能下降投射及其脊髓末端。
影响因子：
--
作者：
Westlund,KN;Bowker,RM;Ziegler,MG;Coulter,JD
通讯作者：
Coulter,JD
血压正常和高血压受试者的血浆 P 物质水平。
影响因子：
12.3
作者：
Campbell,WB;Holland,OB;Gomez-Sanchez,CE;Graham,RM;Pettinger,WA;White,AC
通讯作者：
White,AC
影响因子：
6.1
作者：
HYLDEN, JLK;WILCOX, GL
通讯作者：
WILCOX, GL