Peripheral immunophenotyping of AITD subjects reveals alterations in immune cells in pediatric vs adult-onset AITD.
Peripheral immunophenotyping of AITD subjects reveals alterations in immune cells in pediatric vs adult-onset AITD.
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DOI：
10.1016/j.isci.2021.103626
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发表时间：
2022-01-21
期刊：
影响因子：
5.8
通讯作者：
Smith MJ
中科院分区：
文献类型：
--
作者：
Stensland ZC;Coleman BM;Rihanek M;Baxter RM;Gottlieb PA;Hsieh EWY;Sarapura VD;Simmons KM;Cambier JC;Smith MJ
自身免疫性甲状腺疾病（AITD）是由免疫系统异常激活引起的，允许自身反应性B细胞和T细胞靶向甲状腺导致疾病。虽然AITD在成人中更常被诊断出来，但儿童也会受到影响，但很少被研究。在这里，我们使用细胞计数技术对儿童和成人AITD患者以及年龄匹配的对照组的外周血免疫细胞进行了表型和功能表征。主要研究结果表明，与成人发病的AITD患者不同，与年龄匹配的对照组相比，儿科AITD患者表现出无能B细胞（BND）和DN2 B细胞的减少和未成熟B细胞的增加。这些结果表明，在儿科发病的AITD中看到的外周血免疫细胞的改变可能导致疾病的快速进展。因此，本研究通过显示基于发病年龄的免疫细胞表型和功能差异，证明了AITD的多样性，并可能为未来的治疗提供信息。高危HLA-DR3单倍型在儿童AITD患者中外显率较高，儿童AITD患者自身反应性B细胞亚群频率改变，免疫细胞亚群频率和功能与成人AITD和对照组相似；免疫学方法;细胞生物学
Autoimmune thyroid disease (AITD) is caused by aberrant activation of the immune system allowing autoreactive B and T cells to target the thyroid gland leading to disease. Although AITD is more frequently diagnosed in adults, children are also affected but rarely studied. Here, we performed phenotypic and functional characterization of peripheral blood immune cells from pediatric and adult-onset AITD patients and age-matched controls using mass cytometry. Major findings indicate that unlike adult-onset AITD patients, pediatric AITD patients exhibit a decrease in anergic B cells (BND) and DN2 B cells and an increase in immature B cells compared to age-matched controls. These results indicate alterations in peripheral blood immune cells seen in pediatric-onset AITD could lead to rapid progression of disease. Hence, this study demonstrates diversity of AITD by showing differences in immune cell phenotypes and function based on age of onset, and may inform future therapies. Penetrance of high-risk HLA-DR3 haplotype is higher in pediatric AITD patients Pediatric AITD patients display altered frequency of autoreactive B cell subsets Immune cell subset frequency and function is similar in adult AITD and controls Immunology; Immunological methods; Cell biology
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影响因子：
8.2
作者：
Ekman, Ilse;Ihantola, Emmi-Leena;Kinnunen, Tuure
通讯作者：
Kinnunen, Tuure
影响因子：
5.6
作者：
Fugger, Gernot;Dold, Markus;Kasper, Siegfried
通讯作者：
Kasper, Siegfried
影响因子：
3.7
作者：
Conrad, Valerie K.;Dubay, Christopher J.;Redmond, William L.
通讯作者：
Redmond, William L.
DOI：
10.1084/jem.20080611
发表时间：
2009-01-16
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Duty JA;Szodoray P;Zheng NY;Koelsch KA;Zhang Q;Swiatkowski M;Mathias M;Garman L;Helms C;Nakken B;Smith K;Farris AD;Wilson PC
通讯作者：
Wilson PC
影响因子：
4.4
作者：
Hong, So-Hee;Braley-Mullen, Helen
通讯作者：
Braley-Mullen, Helen