Chemical factors that influence nucleocytoplasmic transport: a fluorescence photobleaching study.
Chemical factors that influence nucleocytoplasmic transport: a fluorescence photobleaching study.
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DOI：
10.1083/jcb.102.3.853
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发表时间：
1986-03
影响因子：
7.8
通讯作者：
SCHINDLER, M
中科院分区：
文献类型：
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作者：
JIANG, LW;SCHINDLER, M
光漂白后荧光重新分布技术用于测量荧光素标记的葡聚糖穿过离体大鼠肝核中核孔复合物的易位率。建立了转运检测系统，可以监测生物活性分子（例如ATP、GTP、cAMP）对易位过程的影响。结果表明，ATP、磷酸肌醇、RNA 和胰岛素可以将转运率从 195% 提高到 432%。进一步证明，伴刀豆球蛋白A（而非小麦胚芽或大豆凝集素）可以完全阻断葡聚糖转运。右旋糖酐转运的效应物与已证明影响 RNA 从分离的细胞核中流出的物质相似。现在提出了一种通过核孔易位的模型，该模型将蛋白质流入和 RNA 流出实验的数据合并到由 ATP 控制的单一途径中。
The technique of fluorescence redistribution after photobleaching was used to measure the translocation rate of fluorescein-labeled dextrans across the nuclear pore complex in isolated rat liver nuclei. A transport assay system was established that could monitor the effect of biologically active molecules, e.g., ATP, GTP, cAMP on the translocation process. The results show that ATP, phosphoinositides, RNA, and insulin can enhance transport rates from 195 to 432%. It was further demonstrated that concanavalin A, but not wheat germ or soybean agglutinin, can block dextran transport completely. The effectors of dextran transport are similar to substances demonstrated to effect the efflux of RNA from isolated nuclei. A model for translocation through the nuclear pore is now presented that incorporates data from protein influx and RNA efflux experiments into a single pathway controlled by ATP.
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影响因子：
64.8
作者：
KALDERON, D;RICHARDSON, WD;SMITH, AE
通讯作者：
SMITH, AE
影响因子：
64.8
作者：
PAINE, PL;MOORE, LC;HOROWITZ, SB
通讯作者：
HOROWITZ, SB
影响因子：
3.7
作者：
SCHINDLER, M
通讯作者：
SCHINDLER, M
影响因子：
2.9
作者：
CLAWSON, GA;JAMES, J;SMUCKLER, EA
通讯作者：
SMUCKLER, EA
DOI：
10.1073/pnas.79.17.5302
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DABAUVALLE, MC;FRANKE, WW
通讯作者：
FRANKE, WW