Formation of large palindromic DNA by homologous recombination of short inverted repeat sequences in Saccharomyces cerevisiae.
Formation of large palindromic DNA by homologous recombination of short inverted repeat sequences in Saccharomyces cerevisiae.
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通过酿酒酵母中短反向重复序列的同源重组形成大回文 DNA。
DOI：
10.1093/genetics/161.3.1065
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发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
Steele,Brandi
中科院分区：
文献类型：
--
作者：
Butler,DavidK;Gillespie,David;Steele,Brandi
大的DNA回文序列在许多真核生物和原核生物基因组中零星地形成，并且通常与扩增的基因相关。DNA双链断裂附近的短反向重复序列的存在与多种生物中大回文的形成有关。以前，我们已经建立了inSaccharomycescerevisaea线性DNA回文有效地形成从一个单拷贝的环状质粒时，DNA双链断裂被引入旁边的一个短的反向重复序列。在这项研究中，我们解决了线性回文形式是否通过分子间反应（即，在头对头排列的两个相同片段之间的反应）或通过不寻常的分子内反应，因为它显然是在回文形成的其他例子。我们的证据支持一个模型，其中回文主要是由一个分子间的反应，涉及短的反向重复序列的同源重组形成。我们还扩大了我们的调查，在回文形成的DNA双链断裂修复基因的要求。我们发现，RAD52基因的缺失显著减少了分子间重组形成的回文，而RAD52上位组中的另外两个基因（RAD51和MRE11）的缺失对回文的形成几乎没有影响。此外，通过核苷酸切除修复基因RAD1的缺失，回文结构的形成显著减少。
Large DNA palindromes form sporadically in many eukaryotic and prokaryotic genomes and are often associated with amplified genes. The presence of a short inverted repeat sequence near a DNA double-strand break has been implicated in the formation of large palindromes in a variety of organisms. Previously we have established that inSaccharomyces cerevisaea linear DNA palindrome is efficiently formed from a single-copy circular plasmid when a DNA double-strand break is introduced next to a short inverted repeat sequence. In this study we address whether the linear palindromes form by anintermolecular reaction (that is, a reaction between two identical fragments in a head-to-head arrangement) or by an unusualintramolecular reaction, as it apparently does in other examples of palindrome formation. Our evidence supports a model in which palindromes are primarily formed by an intermolecular reaction involving homologous recombination of short inverted repeat sequences. We have also extended our investigation into the requirement for DNA double-strand break repair genes in palindrome formation. We have found that a deletion of theRAD52gene significantly reduces palindrome formation by intermolecular recombination and that deletions of two other genes in theRAD52-epistasis group (RAD51andMRE11) have little or no effect on palindrome formation. In addition, palindrome formation is dramatically reduced by a deletion of the nucleotide excision repair geneRAD1.
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影响因子：
14.9
作者：
Josephine Nalbantoglu;M. Meuth
通讯作者：
M. Meuth
两性基因组的组织、生成和复制：综述。
影响因子：
3.5
作者：
E. Rayko
通讯作者：
E. Rayko
影响因子：
56.9
作者：
FISHMANLOBELL, J;HABER, JE
通讯作者：
HABER, JE
影响因子：
64.5
作者：
FORD, M;FRIED, M
通讯作者：
FRIED, M
POL1 的突变增加了酿酒酵母串联反向重复序列的有丝分裂不稳定性。
影响因子：
3.3
作者：
Ruskin,B;Fink,GR
通讯作者：
Fink,GR