Fabrication and characterization of solid-state nanopore arrays for high-throughput DNA sequencing.
Fabrication and characterization of solid-state nanopore arrays for high-throughput DNA sequencing.
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用于高通量DNA测序的固态纳米孔阵列的制造和表征。
DOI：
10.1088/0957-4484/23/38/385308
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发表时间：
2012-09-28
期刊：
影响因子：
3.5
通讯作者：
Meller A
中科院分区：
文献类型：
--
作者：
dela Torre R;Larkin J;Singer A;Meller A
我们报告了均匀尺寸的纳米孔阵列的制造和表征，其集成到用于高通量DNA测序应用的光学检测系统中。使用聚焦离子束研磨，然后使用原子层沉积（ALD）进行TiO 2涂覆来制造纳米孔阵列。TiO 2层将初始孔径减小到低于10 nm的范围，与使用光学读出的基于纳米孔的测序的要求兼容。我们发现，二氧化钛层产生较低的光致发光背景相比，更广泛使用的氧化铝涂层。纳米孔阵列的功能性通过DNA-量子点-缀合物的同时光学检测来证明，所述DNA-量子点-缀合物被电动驱动通过纳米孔。我们的光学方案采用全内反射荧光（TIRF）显微镜照亮了广泛的区域的二氧化钛涂层膜。在实验上实现了一个高度并行的系统，用于观察均匀尺寸的6×6纳米孔阵列中的DNA捕获事件。
We report the fabrication and characterization of uniformly-sized nanopore arrays, integrated into an optical detection system for high-throughput DNA sequencing applications. Nanopore arrays were fabricated using Focused Ion Beam milling followed by TiO2 coating using Atomic Layer Deposition (ALD). The TiO2 layer decreases the initial pore diameter down to sub-10nm range, compatible with the requirements for nanopore-based sequencing using optical readout. We find that the TiO2 layers produce a lower photoluminescence background as compared with the more broadly used Al2O3 coatings. The functionality of the nanopore array was demonstrated by the simultaneous optical detection of DNA-quantum dot-conjugates, which were electro-kinetically driven through the nanopores. Our optical scheme employs Total Internal Reflection Fluorescence (TIRF) microscopy to illuminate a wide area of the TiO2-coated membrane. A highly parallel system for observing DNA capture events in a uniformly-sized 6×6 nanopore array was experimentally realized.
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影响因子：
10.8
作者：
McNally B;Singer A;Yu Z;Sun Y;Weng Z;Meller A
通讯作者：
Meller A
影响因子：
10.8
作者：
Nam, Sung-Wook;Rooks, Michael J.;Rossnagel, Stephen M.
通讯作者：
Rossnagel, Stephen M.
影响因子：
3.3
作者：
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通讯作者：
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影响因子：
64.8
作者：
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通讯作者：
Golovchenko, JA
影响因子：
38.3
作者：
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通讯作者：
Bayley, Hagan