Potential role of autophagy in T‑cell survival in polymyositis and dermatomyositis.
Potential role of autophagy in T‑cell survival in polymyositis and dermatomyositis.
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自噬在多发性肌炎和皮肌炎 T 细胞存活中的潜在作用
DOI：
10.3892/mmr.2017.6693
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发表时间：
2017-08
影响因子：
3.4
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Shu X;Chen F;Peng Q;Lu X;Tian X;Wang Y;Wang G
先前已在多发性肌炎/皮肌炎 (PM/DM) 患者中发现外周血 T 淋巴细胞减少症。因此，本研究旨在探讨自噬在 PM/DM 患者外周血 T 细胞存活中的潜在作用。采用透射电镜检测 24 名 PM/DM 患者和 21 名健康对照者外周血分化簇 (CD)3+ T 细胞自噬体的形成情况。分别通过蛋白质印迹分析和逆转录定量聚合酶链反应检测自噬相关分子的蛋白质和mRNA表达水平。 PM/DM患者外周血CD3+T细胞数量显着减少。与健康对照相比，PM/DM 患者的 CD3+ T 细胞凋亡中位百分比显着增加。此外，与健康对照相比，PM/DM 患者循环 CD3+ T 细胞中自噬体的数量以及自噬标记物微管相关蛋白 1A/1B-轻链 3 (LC3) 和 Beclin-1 的表达显着减少。 PM/DM 患者中 LC3 和 Beclin-1 蛋白水平与循环 CD3+ T 细胞凋亡率呈负相关。与未治疗的细胞相比，接受雷帕霉素治疗的 PM/DM 患者的 CD3+ T 细胞自噬增加，细胞凋亡减少 (P<0.05)。总之，这些结果表明自噬可能对 PM/DM 患者的外周血 T 细胞具有潜在的保护作用。
Peripheral blood T lymphocytopenia has previously been identified in polymyositis/dermatomyositis (PM/DM) patients. Therefore, the present study aimed to examine the potential role of autophagy in peripheral blood T cell survival in PM/DM patients. Transmission electron microscopy was used to detect the formation of autophagosomes of peripheral blood cluster of differentiation (CD)3+ T cells obtained from 24 patients with PM/DM and 21 healthy controls. Protein and mRNA expression levels of autophagy-related molecules were examined by western blot analysis and reverse transcription-quantitative polymerase chain reaction, respectively. The number of peripheral blood CD3+ T cells decreased significantly in PM/DM patients. The median percentage of apoptosis of CD3+ T cells in PM/DM patients was significantly increased compared with healthy controls. Furthermore, the number of autophagosomes and the expression of the autophagy markers microtubule-associated protein 1A/1B-light chain 3 (LC3) and Beclin-1 were significantly reduced in the circulating CD3+ T cells of PM/DM patients compared with those of healthy controls. LC3 and Beclin-1 protein levels correlated negatively with apoptosis rates in circulating CD3+ T cells in patients with PM/DM. CD3+ T cells from PM/DM patients treated with rapamycin increased autophagy and decreased apoptosis compared with untreated cells (P<0.05). In conclusion, these results suggested that autophagy may serve a potential protective role in the peripheral blood T cells of patients with PM/DM.
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影响因子：
1.2
作者：
Ishii, Wataru;Matsuda, Masayuki;Ikeda, Shu-ichi
通讯作者：
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影响因子：
30.8
作者：
Han, Jian-Wen;Zheng, Hou-Feng;Zhang, Xue-Jun
通讯作者：
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影响因子：
4.8
作者：
Alessandri, Cristiano;Barbati, Cristiana;Pierdominici, Marina
通讯作者：
Pierdominici, Marina
影响因子：
13.3
作者：
Gros F;Arnold J;Page N;Décossas M;Korganow AS;Martin T;Muller S
通讯作者：
Muller S
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影响因子：
7.3
作者：
Bhattacharya A;Eissa NT
通讯作者：
Eissa NT