Genome-wide association studies identify heavy metal ATPase3 as the primary determinant of natural variation in leaf cadmium in Arabidopsis thaliana.
Genome-wide association studies identify heavy metal ATPase3 as the primary determinant of natural variation in leaf cadmium in Arabidopsis thaliana.
复制标题
DOI：
10.1371/journal.pgen.1002923
复制
发表时间：
2012-09
期刊：
影响因子：
4.5
通讯作者：
Salt DE
中科院分区：
文献类型：
--
作者：
Chao DY;Silva A;Baxter I;Huang YS;Nordborg M;Danku J;Lahner B;Yakubova E;Salt DE
了解镉在植物中的积累机制对于降低镉通过饮食和环境暴露对植物和人类的潜在毒性具有重要意义。在这里，我们报告了一项研究，以揭示镉积累的自然变异的遗传基础，在世界范围内收集的349个野生拟南芥种质。当这些品种在一个受控的普通花园中生长时，我们确定了叶片镉积累量的4倍变化（0.5-2 µg Cd g−1干重）。通过结合全基因组关联作图、F2实验群体的连锁作图和转基因互补，我们揭示了HMA 3是唯一的主要位点，负责我们在这个不同的A. thaliana加入对149株A.拟南芥种质揭示了10种主要的天然蛋白质单倍型的存在。这些单倍型与叶镉积累和遗传互补实验的关联表明，这些单倍型中有5个是活跃的，5个是不活跃的，并且叶镉积累升高与由无义突变和改变两个特定氨基酸的多态性引起的HMA 3功能降低有关。镉（Cd）是一种潜在的有毒金属污染物，在世界许多地区威胁着食品质量和人类健康。植物已经进化出从土壤中获取锌和铁等必需金属的机制。虽然往往是相当具体的，这种机制也可以导致镉的积累植物。了解导致粮食作物中镉积累的过程中的自然变化有助于最大限度地减少对人类健康造成的风险。我们已经发现，在一个单一的基因，编码重金属ATP酶3（HMA 3）的DNA序列的变化，驱动镉积累的变化，我们观察到在世界范围内的样本拟南芥。我们确定了10个主要的HMA 3蛋白变体，其中5个有助于减少镉在A. thaliana.
Understanding the mechanism of cadmium (Cd) accumulation in plants is important to help reduce its potential toxicity to both plants and humans through dietary and environmental exposure. Here, we report on a study to uncover the genetic basis underlying natural variation in Cd accumulation in a world-wide collection of 349 wild collected Arabidopsis thaliana accessions. We identified a 4-fold variation (0.5–2 µg Cd g−1 dry weight) in leaf Cd accumulation when these accessions were grown in a controlled common garden. By combining genome-wide association mapping, linkage mapping in an experimental F2 population, and transgenic complementation, we reveal that HMA3 is the sole major locus responsible for the variation in leaf Cd accumulation we observe in this diverse population of A. thaliana accessions. Analysis of the predicted amino acid sequence of HMA3 from 149 A. thaliana accessions reveals the existence of 10 major natural protein haplotypes. Association of these haplotypes with leaf Cd accumulation and genetics complementation experiments indicate that 5 of these haplotypes are active and 5 are inactive, and that elevated leaf Cd accumulation is associated with the reduced function of HMA3 caused by a nonsense mutation and polymorphisms that change two specific amino acids. Cadmium (Cd) is a potentially toxic metal pollutant that threatens food quality and human health in many regions of the world. Plants have evolved mechanisms for the acquisition of essential metals such as zinc and iron from the soil. Though often quite specific, such mechanisms can also lead to the accumulation of Cd by plants. Understanding natural variation in the processes that contribute to Cd accumulation in food crops could help minimize the human health risk posed. We have discovered that DNA sequence changes at a single gene, which encodes the Heavy Metal ATPase 3 (HMA3), drives the variation in Cd accumulation we observe in a world-wide sample of Arabidopsis thaliana. We identified 10 major HMA3 protein variants, of which five contribute to reduce Cd accumulation in leaves of A. thaliana.
登录
查看更多内容
影响因子：
4.5
作者：
Baxter I;Brazelton JN;Yu D;Huang YS;Lahner B;Yakubova E;Li Y;Bergelson J;Borevitz JO;Nordborg M;Vitek O;Salt DE
通讯作者：
Salt DE
影响因子：
13.8
作者：
Korenkov, Victor;King, Brian;Wagner, George J.
通讯作者：
Wagner, George J.
影响因子：
4.5
作者：
Filiault DL;Maloof JN
通讯作者：
Maloof JN
影响因子：
4.5
作者：
Brachi B;Faure N;Horton M;Flahauw E;Vazquez A;Nordborg M;Bergelson J;Cuguen J;Roux F
通讯作者：
Roux F
拟南芥中全基因组的关联映射确定了先前已知的开花时间和病原体抗性基因。
影响因子：
4.5
作者：
Aranzana MJ;Kim S;Zhao K;Bakker E;Horton M;Jakob K;Lister C;Molitor J;Shindo C;Tang C;Toomajian C;Traw B;Zheng H;Bergelson J;Dean C;Marjoram P;Nordborg M
通讯作者：
Nordborg M