Efficient Hydrogen-Dependent Carbon Dioxide Reduction by Escherichia coli.
Efficient Hydrogen-Dependent Carbon Dioxide Reduction by Escherichia coli.
复制标题
大肠杆菌的有效二氧化碳还原二氧化碳。
DOI：
10.1016/j.cub.2017.11.050
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发表时间：
2018-01-08
期刊：
影响因子：
--
通讯作者：
Sargent F
中科院分区：
文献类型：
--
作者：
Roger M;Brown F;Gabrielli W;Sargent F
二氧化碳的氢依赖性还原为甲酸为温室气体封存、碳减排技术、氢运输和储存以及可再生化学原料的可持续生产提供了一条有前途的途径。将CO2直接氢化为甲酸盐的最常见方法是在均相或非均相反应中使用化学催化剂。另一种方法是利用生物体的能力来进行生物反应。然而，尽管CO2固定途径在自然界中广泛分布，但仅描述了少数具有进行CO2直接氢化的能力的酶。来自大肠杆菌的甲酸氢裂解酶（FHL）通常将甲酸氧化成二氧化碳，并将该反应直接与质子还原成分子氢偶联。在这项工作中，FHL的逆反应被解锁。已经确定，当气态CO2和H2处于压力下（高达10 bar）时，FHL可以作为高效的氢依赖性二氧化碳还原酶运行。使用完整的全细胞，观察到加压系统将100%的气态CO2快速转化为甲酸，并且观察到>500 mM甲酸盐在溶液中积累。利用逆反应有可能使多功能的大肠杆菌系统被用作一种令人兴奋的新碳捕获技术，或者作为一种专门用于甲酸生产的细胞工厂，甲酸本身就是一种商品，也是合成其他有价值化学品的原料。大肠杆菌产生甲酸氢解酶（FHL）FHL可以在两种模式下发挥作用，这取决于主要的环境条件加压的CO2和H2允许FHL作为氢依赖性CO2还原酶发挥作用所产生的甲酸在细菌细胞外积累在厌氧条件下，大肠杆菌产生甲酸氢解酶（FHL）。Roger等人揭示了当H2和CO2气体被置于增加的压力下时，FHL作为一种有效的氢依赖性二氧化碳还原酶起作用。反应的产物是甲酸，它在细菌细胞外积累。
Hydrogen-dependent reduction of carbon dioxide to formic acid offers a promising route to greenhouse gas sequestration, carbon abatement technologies, hydrogen transport and storage, and the sustainable generation of renewable chemical feedstocks. The most common approach to performing direct hydrogenation of CO2 to formate is to use chemical catalysts in homogeneous or heterogeneous reactions. An alternative approach is to use the ability of living organisms to perform this reaction biologically. However, although CO2 fixation pathways are widely distributed in nature, only a few enzymes have been described that have the ability to perform the direct hydrogenation of CO2. The formate hydrogenlyase (FHL) enzyme from Escherichia coli normally oxidizes formic acid to carbon dioxide and couples that reaction directly to the reduction of protons to molecular hydrogen. In this work, the reverse reaction of FHL is unlocked. It is established that FHL can operate as a highly efficient hydrogen-dependent carbon dioxide reductase when gaseous CO2 and H2 are placed under pressure (up to 10 bar). Using intact whole cells, the pressurized system was observed to rapidly convert 100% of gaseous CO2 to formic acid, and >500 mM formate was observed to accumulate in solution. Harnessing the reverse reaction has the potential to allow the versatile E. coli system to be employed as an exciting new carbon capture technology or as a cell factory dedicated to formic acid production, which is a commodity in itself as well as a feedstock for the synthesis of other valued chemicals. Escherichia coli produces a formate hydrogenlyase (FHL) enzyme FHL can function in two modes dependent on the prevailing environmental conditions Pressurized CO2 and H2 allow FHL to function as a hydrogen-dependent CO2 reductase The produced formic acid accumulates outside of the bacterial cells Under anaerobic conditions, Escherichia coli produces a formate hydrogenlyase (FHL) enzyme. Roger et al. reveal that when H2 and CO2 gases are placed under increasing pressure, FHL operates as an efficient hydrogen-dependent carbon dioxide reductase. The product of the reaction is formic acid, which accumulates outside the bacterial cells.
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影响因子：
4.8
作者：
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通讯作者：
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DOI：
10.1073/pnas.76.9.4530
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