Cytokine expression in rat molar gingival periodontal tissues after topical application of lipopolysaccharide
Cytokine expression in rat molar gingival periodontal tissues after topical application of lipopolysaccharide
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局部应用脂多糖后大鼠磨牙牙龈牙周组织细胞因子的表达
DOI：
--
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发表时间：
2001
影响因子：
2.3
通讯作者：
T. Takata
中科院分区：
文献类型：
--
作者：
M. Miyauchi;Sunao Sato;S. Kitagawa;M. Hiraoka;Y. Kudo;I. Ogawa;Ming Zhao;T. Takata
抽象的。众所周知，宿主细胞产生的促炎细胞因子在牙周组织破坏中起着重要作用。然而，细胞因子在牙周病发生发展过程中在活体牙周组织中的定位尚不清楚。采用免疫组织化学方法检测大鼠磨牙牙龈沟局部注射脂多糖后1h、3h、1d、2d、3d、7d的致炎细胞因子IL-1α、IL-1β、肿瘤坏死因子-α的表达。在正常牙周组织中，连接上皮(JE)、口腔沟和口腔牙龈上皮内可见少量细胞因子阳性的上皮细胞，连接下上皮区有巨噬细胞浸润，成骨细胞排列在牙槽骨表面。残留的牙周膜上皮对IL-1β呈强阳性反应，而对其他两种细胞因子呈阴性反应。在内毒素处理后3h，JE内几乎所有细胞都呈强阳性反应。此外，细胞因子阳性细胞，包括中性粒细胞、巨噬细胞和成纤维细胞，也可见于结缔组织中。术后第2天，JE中细胞因子表达逐渐减少，结缔组织中细胞因子阳性细胞增多。细胞因子阳性染色见于破骨细胞和破骨前细胞，这些细胞沿牙槽骨缘出现。这些结果表明，除巨噬细胞、中性粒细胞和成纤维细胞外，JE细胞还是对内毒素刺激反应的肿瘤坏死因子-α、IL-1α和IL-1β的有效来源，提示JE细胞可能在对抗内毒素攻击的第一道防线中发挥重要作用，各种宿主细胞瞬时产生的促炎细胞因子可能参与了炎症的启动和随后的牙周组织破坏。
Abstract. It is well known that proinflammatory cytokines produced by host cells play an important role in periodontal tissue destruction. However, the localization of the cytokines in in vivo periodontal tissues during development of periodontal disease has not been determined. Immunohistochemical expression of proinflammatory cytokines including IL-1α, IL-1β, and TNF-α was examined at 1 and 3 h, and 1, 2, 3, and 7 days after topical application of lipopolysaccharide (LPS; 5 mg/ml in physiological saline) from E. coli into the rat molar gingival sulcus. In the normal periodontal tissues, a small number of cytokine-positive epithelial cells were seen in the junctional epithelium (JE), oral sulcular and oral gingival epithelium, in addition to macrophages infiltrating in the subjunctional epithelial area and osteoblasts lining the alveolar bone surface. Epithelial remnants of Malassez existing throughout periodontal ligament were intensely positive for IL-1β but negative for the other two cytokines. At 3 h after the LPS treatment, almost all cells in the JE were strongly positive for the cytokines examined. In addition, several cytokine-positive cells, including neutrophils, macrophages, and fibroblasts, were seen in the subjunctional epithelial connective tissue. At day 2, expression of the cytokines in the JE gradually decreased, while cytokine-positive cells in the connective tissue increased in number. Positive staining of the cytokines was seen in osteoclasts and preosteoclasts which appeared along the alveolar bone margin in this period. The number of cytokine-positive cells decreased by day 7. These findings indicate that, in addition to macrophages, neutrophils, and fibroblasts, the JE cells are a potent source of TNF-α, IL-1α, and IL-1β reacting to LPS application, and suggest that JE cells may play an important role in the first line of defense against LPS challenge, and the proinflammatory cytokines transiently produced by various host cells may be involved in the initiation of inflammation and subsequent periodontal tissue destruction.
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影响因子：
6.7
作者：
GOODSON, JM;TANNER, ACR;SOCRANSKY, SS
通讯作者：
SOCRANSKY, SS
Interleukin-1 beta 在体内刺激骨吸收并抑制骨形成。
DOI：
--
发表时间：
1991
期刊：
Lymphokine and cytokine research
影响因子：
--
作者：
Nguyen,L;Dewhirst,FE;Hauschka,PV;Stashenko,P
通讯作者：
Stashenko,P
影响因子：
3.5
作者：
PAGE, RC
通讯作者：
PAGE, RC
影响因子：
15.9
作者：
JUNG, HC;ECKMANN, L;KAGNOFF, MF
通讯作者：
KAGNOFF, MF
影响因子：
4.3
作者：
STASHENKO, P;JANDINSKI, JJ;SOCRANSKY, SS
通讯作者：
SOCRANSKY, SS