Mapping the route from naive pluripotency to lineage specification.
Mapping the route from naive pluripotency to lineage specification.
复制标题
DOI：
10.1098/rstb.2013.0540
复制
发表时间：
2014-12-05
期刊：
影响因子：
--
通讯作者：
Smith A
中科院分区：
文献类型：
--
作者：
Kalkan T;Smith A
在小鼠囊胚中，外胚层细胞在着床前不久刚刚形成。它们具有独特的发育可塑性，称为幼稚多能性。为了继续发育，这种幼稚状态必须在植入后72小时内被多谱系分化所包含。在受控条件下培养的原始胚胎干细胞（ESCs）的体外分化为解剖和理解从原始多能性退出和进入谱系规范的过程提供了一个易于处理的系统。在最近的大规模RNAi和诱变筛选中，该系统的开发已经发现了多个新的因素和模块，这些因素和模块驱动或促进了幼稚状态的进展。值得注意的是，这些研究表明，控制原始状态的转录因子网络在谱系规范标记上调之前被迅速拆除，创造了我们称之为形成性多能性的中间状态。在这里，我们总结了这些发现，并提出了ESC分化状态转变的路线图，该路线图反映了胚胎中外胚层进展的有序动态。
In the mouse blastocyst, epiblast cells are newly formed shortly before implantation. They possess a unique developmental plasticity, termed naive pluripotency. For development to proceed, this naive state must be subsumed by multi-lineage differentiation within 72 h following implantation. In vitro differentiation of naive embryonic stem cells (ESCs) cultured in controlled conditions provides a tractable system to dissect and understand the process of exit from naive pluripotency and entry into lineage specification. Exploitation of this system in recent large-scale RNAi and mutagenesis screens has uncovered multiple new factors and modules that drive or facilitate progression out of the naive state. Notably, these studies show that the transcription factor network that governs the naive state is rapidly dismantled prior to upregulation of lineage specification markers, creating an intermediate state that we term formative pluripotency. Here, we summarize these findings and propose a road map for state transitions in ESC differentiation that reflects the orderly dynamics of epiblast progression in the embryo.
登录
查看更多内容
影响因子：
21.3
作者：
Boroviak T;Loos R;Bertone P;Smith A;Nichols J
通讯作者：
Nichols J
影响因子：
9.2
作者：
Chen, Dong;Zheng, Wei;Lin, Aiping;Uyhazi, Katherine;Zhao, Hongyu;Lin, Haifan
通讯作者：
Lin, Haifan
影响因子：
64.8
作者：
Brons, I. Gabrielle M.;Smithers, Lucy E.;Vallier, Ludovic
通讯作者：
Vallier, Ludovic
影响因子：
10.5
作者：
Cole, Megan F.;Johnstone, Sarah E.;Young, Richard A.
通讯作者：
Young, Richard A.
影响因子：
2.6
作者：
Haffner-Krausz, R;Gorivodsky, M;Lonai, P
通讯作者：
Lonai, P