Expression and role of E- and P-cadherin adhesion molecules in embryonic histogenesis. II. Skin morphogenesis.-猫眼课题宝

E-和P-钙粘蛋白粘附分子在胚胎组织发生中的表达和作用。

DOI：

发表时间：

1989

期刊：

Development

影响因子：

4.6

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

Yohei Hirai;A. Nose;Shigeyasu Kobayashi;Masatoshi Takeichi

以小鼠上唇皮肤为研究对象，研究了E-钙粘附素和P-钙粘附素在表皮和毛囊组织发生中的表达和作用。P-钙粘附素仅在这些组织的增殖区表达，即在表面表皮的萌发层、外根鞘和毛发基质中表达。E-钙粘附素在这些层中共表达，但该分子也在非增殖区表达，如表层表皮的中间层和内根鞘的未成熟区。P-钙粘附素和E-钙粘附素在表皮角质层、外根鞘最外层和成熟毛纤维中均未检测到。在真皮细胞中未检测到这两种钙粘附素。我们在没有或存在抗E-钙粘蛋白(ECCD-1)或抗P-钙粘蛋白(PCD-1)的单抗的情况下，体外培养上唇皮肤。在对照培养中，皮肤形态发生通常发生在毛囊生长和真皮细胞浓缩形成真皮鞘的模式中。然而，ECCD-1和PCD-1的混合物在几个方面诱导了皮肤的异常形态发生。(1)基底上皮细胞立方或柱状排列扭曲。(2)毛囊变形。(3)抑制真皮细胞凝聚，使真皮细胞分布均匀。这些结果表明，存在于表皮细胞中的钙粘附素不仅参与维持这些细胞的排列，而且还参与诱导真皮凝结。

Expression and the role of E- and P-cadherin in the histogenesis of the surface epidermis and hair follicles were examined using the upper lip skin of the mouse. P-cadherin is expressed exclusively in the proliferating region of these tissues, that is in the germinative layer of the surface epidermis, the outer root sheath and the hair matrix. E-cadherin is coexpressed in these layers but this molecule was also detected in non-proliferating regions such as the intermediate layer of the surface epidermis and the immature regions of the inner root sheath. Neither P- nor E-cadherin was detected in fully keratinized layers such as the horny layer of the surface epidermis, the outermost layer of the outer root sheath and the mature hair fibres. These two cadherins were not detected in dermal cells. We cultured pieces of the upper lip skin in vitro in the absence or presence of a monoclonal antibody to E-cadherin (ECCD-1) or to P-cadherin (PCD-1). In control cultures, skin morphogenesis normally occurred in a pattern whereby the hair follicles grew and dermal cells were condensed to form the dermal sheath. A mixture of ECCD-1 and PCD-1, however, induced abnormal morphogenesis in the skin in several respects. (1) The cuboidal or columnar arrangement of basal epithelial cells was distorted. (2) Hair follicles were deformed. (3) Condensation of dermal cells was suppressed, causing a homogeneous distribution of these cells. These results suggest that cadherins present in epidermal cells are involved not only in maintaining the arrangement of these cells but also in inducing dermal condensation.

Antibodies to liver cell adhesion molecule perturb inductive interactions and alter feather pattern and structure.