Bioactive glass promotes the barrier functional behaviors of keratinocytes and improves the Re-epithelialization in wound healing in diabetic rats.
Bioactive glass promotes the barrier functional behaviors of keratinocytes and improves the Re-epithelialization in wound healing in diabetic rats.
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DOI：
10.1016/j.bioactmat.2021.02.041
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发表时间：
2021-10
影响因子：
18.9
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Tang F;Li J;Xie W;Mo Y;Ouyang L;Zhao F;Fu X;Chen X
在皮肤损伤后，必须迅速启动再上皮化，以恢复表皮的完整性和屏障功能。然而，在糖尿病足部溃疡等慢性伤口中，这一过程往往会延迟或中断。考虑到BG颗粒可以激活不同细胞中的多个基因，我们推测生物活性玻璃(BG)可能能够调节角质形成细胞的屏障功能行为。通过测量跨皮电阻(TEER)和细胞旁示踪剂流量，我们发现58S-BG提取物显著增强了角质形成细胞单层的屏障功能。BG提取物可能通过促进角质形成细胞分化和紧密连接的形成而发挥这种作用，关键分化标志物(K10和包衣蛋白)和TJ蛋白claudin-1的表达增加，以及四种主要TJ蛋白(claudin-1、occludin、Jam-A和ZO-1)的亚细胞位置发生改变。此外，细胞划痕实验显示，BG提取物诱导了角质形成细胞的集体迁移，但与对照组相比，它们并没有加快迁移速度。用糖尿病大鼠创伤模型进行的体内研究表明，BG提取物加速了再上皮化过程，刺激了角质形成细胞的分化，并促进了新生表皮紧密连接的形成。我们的发现揭示了BGS对角质形成细胞的重要作用，并强调了它通过有效地恢复创伤皮肤的屏障功能而在慢性伤口愈合中的潜在应用。慢性创面的再上皮化常常被延迟或中断。58S-BG提取物诱导角质形成细胞的集体迁移。58S-BG提取物通过促进角质形成细胞分化和紧密连接的形成，调节角质形成细胞的屏障功能行为。
Upon skin injury, re-epithelialization must be triggered promptly to restore the integrity and barrier function of the epidermis. However, this process is often delayed or interrupted in chronic wounds like diabetic foot ulcers. Considering that BG particles can activate multiple genes in various cells, herein, we hypothesized that bioactive glass (BG) might be able to modulate the barrier functional behaviors of keratinocytes. By measuring the transepithelial electrical resistance (TEER) and the paracellular tracer flux, we found the 58S-BG extracts substantially enhanced the barrier function of keratinocyte monolayers. The BG extracts might exert such effects by promoting the keratinocyte differentiation and the formation of tight junctions, as evidenced by the increased expression of critical differentiation markers (K10 and involucrin) and TJ protein claudin-1, as well as the altered subcellular location of four major TJ proteins (claudin-1, occludin, JAM-A, and ZO-1). Besides, the cell scratch assay showed that BG extracts induced the collective migration of keratinocytes, though they did not accelerate the migration rate compared to the control. The in vivo study using a diabetic rat wound model demonstrated that the BG extracts accelerated the process of re-epithelialization, stimulated keratinocyte differentiation, and promoted the formation of tight junctions in the newly regenerated epidermis. Our findings revealed the crucial effects of BGs on keratinocytes and highlighted its potential application for chronic wound healing by restoring the barrier function of the wounded skin effectively. Re-epithelialization is often delayed or interrupted in chronic wounds The 58S-BG extracts induced the collective migration of keratinocytes. The 58S-BG extracts regulated the barrier functional behaviors of keratinocytes by promoting the keratinocyte differentiation and the formation of tight junctions.
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DOI：
10.1080/19381980.2016.1267078
发表时间：
2017
期刊：
Dermato-endocrinology
影响因子：
--
作者：
Leguina-Ruzzi A;Valderas JP
通讯作者：
Valderas JP
DOI：
10.1177/2211068214561025
发表时间：
2015-04
期刊：
Journal of laboratory automation
影响因子：
--
作者：
Srinivasan B;Kolli AR;Esch MB;Abaci HE;Shuler ML;Hickman JJ
通讯作者：
Hickman JJ
紧密连接蛋白 Occludin 调节上皮细胞的定向迁移。
影响因子：
11.8
作者：
Du, Dan;Xu, Feilai;Chen, Zhengjun
通讯作者：
Chen, Zhengjun
影响因子：
3.2
作者：
Usui, Marcia L.;Mansbridge, Jonathan N.;Olerud, John E.
通讯作者：
Olerud, John E.
影响因子：
2.6
作者：
Kim, Kyeong Ah;Jung, Joo Hyun;Kim, Seon Tae
通讯作者：
Kim, Seon Tae