A biochemical method for distinguishing between the sexes of fishes by the presence of yolk protein in the blood
A biochemical method for distinguishing between the sexes of fishes by the presence of yolk protein in the blood
复制标题
通过血液中蛋黄蛋白的存在来区分鱼类性别的生化方法
DOI：
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
S. M. Harvey
中科院分区：
文献类型：
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作者：
J. Craik;S. M. Harvey
描述了一种通过对血液样本进行生化测试来区分海洋硬骨鱼和淡水硬骨鱼的成年雌性硬骨鱼的方法。这项测试依赖于血浆中碱性不稳定蛋白连接磷的测量，这是卵黄蛋白(卵黄蛋白原)的一种特定测量方法。在卵黄形成雌性鱼中，通常发现20-100μg蛋白质磷和血浆−-1的值，而在雄性、非卵黄生成雌性鱼和未成熟的雌鱼中，该值通常为7.5，通常为5μg ml−1。在适当的季节，大多数雌性鱼可以被确认。在产卵前2-3个月可获得良好的结果，某些物种在产卵后的一段时间内也能获得良好的效果。与放射免疫法和免疫电泳法等免疫学技术相比，该方法的优点是可适用的脊椎动物种类广泛，而且成本低。它的缺点是灵敏度较低，且必须使用较大的血浆体积(0.5ml)。
A method is described by which the adult females of marine and freshwater teleosts can be distinguished by a biochemical test performed on a blood sample. The test depends on the measurement in the blood plasma of alkali-labile protein-linked phosphorus, a specific measure of yolk protein (vitellogenin). In vitellogenic females, values of 20–100 μg protein phosphorus ml plasma−1 are usually found, while in males, non-vitellogenic females and immature fish of both sexes the value is <7.5 and usually <5 μg ml−1. At the appropriate season, most females can be positively identified. Good results can be obtained 2–3 months before spawning, and in some species for an undefined period after spawning. The advantages of this method over immunological techniques for the determination of vitellogenin, such as radioimmunoassay and immunoelectrophoresis, are the wide range of vertebrate species to which it can be applied, and its low cost. Its disadvantages are its lower sensitivity and the larger volume of plasma (0.5 ml) which must be used.
他莫昔芬诱导禽血清载脂蛋白 II 和卵黄蛋白原。
DOI：
10.1016/0006-291x(81)91180-3
发表时间：
1981
影响因子：
3.1
作者：
Blue,ML;Williams,DL
通讯作者：
Williams,DL