The Arabidopsis MATERNAL EFFECT EMBRYO ARREST45 protein modulates maternal auxin biosynthesis and controls seed size by inducing AINTEGUMENTA.

The Arabidopsis MATERNAL EFFECT EMBRYO ARREST45 protein modulates maternal auxin biosynthesis and controls seed size by inducing AINTEGUMENTA.
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拟南芥母体效应胚胎抑制45蛋白调节母体生长素生物合成并通过诱导AINTEGUMENTA控制种子大小

DOI:
10.1093/plcell/koab084
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发表时间:
2021-07-19
期刊:
The Plant cell
影响因子:
--
通讯作者:
Su YH
Su YH
中科院分区:
其他
文献类型:
--
作者:
Li YJ;Yu Y;Liu X;Zhang XS;Su YH

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母本组织中的MEE 45-ANT途径调节拟南芥生长素生物合成和种子大小。种子大小是决定作物产量的一个主要因素,它是通过母体组织和合子组织的协调发育来控制的。在这里,我们确定了拟南芥母本效应胚胎阻滞45(MEE 45)作为B3转录因子,控制细胞增殖和母系调节种子大小通过其转录激活AINTEGUMENTA(ANT)和其下游控制生长素的生物合成在胚珠珠被。在描述了mee 45突变体中减少的种子和器官大小表型并发现MEE 45的过表达导致过大的种子后,我们发现MEE 45蛋白可以结合ANT基因座的启动子区域并正向调节其转录。ANT反过来激活胚珠珠被中生长素生物合成基因(例如YUCCA 4)的表达。因此,我们的研究结果说明了母体组织介导的种子大小调节的机制,并表明MEE 45及其下游成分可以用于开发高产作物品种。
A MEE45–ANT pathway in maternal tissues modulates auxin biosynthesis and seed size in Arabidopsis. Seed size is a major factor determining crop yields that is controlled through the coordinated development of maternal and zygotic tissues. Here, we identified Arabidopsis MATERNAL EFFECT EMBRYO ARREST45 (MEE45) as a B3 transcription factor that controls cell proliferation and maternally regulates seed size through its transcriptional activation of AINTEGUMENTA (ANT) and its downstream control of auxin biosynthesis in the ovule integument. After characterizing reduced seed and organ size phenotypes in mee45 mutants and finding that overexpression of MEE45 causes oversized seeds, we discovered that the MEE45 protein can bind to the promoter region of the ANT locus and positively regulate its transcription. ANT in-turn activates the expression of auxin biosynthetic genes (e.g. YUCCA4) in the ovule integument. Our results thus illustrate mechanisms underlying maternal tissue-mediated regulation of seed size and suggest that MEE45 and its downstream components can be harnessed to develop higher-yielding crop varieties.
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