Behavioral characterization of withdrawal following chronic voluntary ethanol consumption via intermittent two-bottle choice points to different susceptibility categories.
Behavioral characterization of withdrawal following chronic voluntary ethanol consumption via intermittent two-bottle choice points to different susceptibility categories.
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DOI：
10.1111/acer.14785
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发表时间：
2022-04
期刊：
影响因子：
--
通讯作者：
De Biasi, Mariella
中科院分区：
文献类型：
--
作者：
Carde, Natalia A. Quijano;De Biasi, Mariella
关键词：
Alcohol is one of the most commonly abused drugs worldwide. Cessation from chronic alcohol consumption can result in the presentation of withdrawal symptoms that commonly promote relapse in individuals with alcohol use disorder (AUD). Thus, preclinical models of voluntary alcohol consumption in which animals manifest spontaneous signs of withdrawal after alcohol cessation can be useful to study AUD and its treatment. The intermittent two-bottle choice paradigm (I2BC) has been extensively used to examine alcohol intake in rodents. However, previous studies have reported conflicting observations regarding its potential to result in the spontaneous manifestation of withdrawal upon alcohol cessation. We employed a battery of behavioral tests to examine the emergence of affective and physical signs of withdrawal in female and male mice exposed to ethanol in the I2BC for 10 weeks. Specifically, mice of both sexes undergoing 24-h withdrawal from the I2BC were tested for physical signs of withdrawal, anxiety-like behavior in the open field arena (OFA) and elevated plus maze (EPM), and anxiety/compulsive-like behavior in the marble burying test (MBT). The main outcomes from these tests were combined into a behavioral severity score to describe the overall behavioral phenotype. Both female and male mice undergoing withdrawal from the I2BC displayed elevated physical signs of withdrawal, and anxiety-associated behavior in the EPM and MBT. Analysis of the overall behavioral severity revealed more severe phenotypes in female and male mice undergoing withdrawal from the I2BC compared to controls. Additionally, stratification of the subjects based on severity scores demonstrated differential distribution of severities between the exposure groups. Overall, we confirmed that a significant fraction of mice chronically exposed to alcohol in the I2BC display spontaneous withdrawal, and that computing a severity score from a combination of behavioral metrics can be a useful approach for pre-clinical research to model evaluation tools used in patients with AUD.
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DOI：
10.1111/j.1601-183x.2009.00476.x
发表时间：
2009-06
期刊：
Genes, brain, and behavior
影响因子：
--
作者：
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De Biasi M
影响因子：
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影响因子：
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通讯作者：
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影响因子：
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DOI：
10.1007/7854_2011_162
发表时间：
2013-01-01
期刊：
BEHAVIORAL NEUROBIOLOGY OF ALCOHOL ADDICTION
影响因子：
--
作者：
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通讯作者：
Hitzemann, Robert J.