Dynamic Responses of Ascorbate Pool and Metabolism in Lettuce to Long-term Continuous Light Provided by Red and Blue LEDs
Dynamic Responses of Ascorbate Pool and Metabolism in Lettuce to Long-term Continuous Light Provided by Red and Blue LEDs
复制标题
生菜中抗坏血酸库和代谢对红色和蓝色 LED 提供的长期连续光的动态响应
DOI：
10.1016/j.envexpbot.2019.04.003
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发表时间：
2019-07
影响因子：
5.7
通讯作者：
Liu Wenke
中科院分区：
文献类型：
--
作者：
Zha Lingyan;Zhang Yubin;Liu Wenke
为了解长时间红蓝连续光照（CL, 24/0 h, 200 μmol·m−2·s−1）下生菜生长、抗坏血酸库和代谢的动态变化，在15 d内每隔3 d对生菜生长参数、抗坏血酸库大小以及参与抗坏血酸代谢的关键酶的活性和基因表达水平进行了研究。与正常光周期（对照，16/8 h, 200 μmol·m−2·s−1）相比，CL处理下生菜植株获得了更多的鲜重和干物质，比叶重比叶面积的影响大于比叶面积。CL显著提高了还原型抗坏血酸（AsA）和脱氢抗坏血酸（DHA）含量，并在试验期间保持相对稳定的水平。AsA和DHA含量的持续增加分别归因于l -半乳糖-1,4-内酯脱氢酶和抗坏血酸过氧化物酶活性的上调，而不是它们的基因表达。虽然CL提高了谷胱甘肽含量和谷胱甘肽还原酶活性，但由于脱氢抗坏血酸还原酶的活性和表达水平与对照组相似，它们对AsA再生的贡献有限。此外，与对照相比，CL处理下h2o2含量也明显升高，但从第6天开始就维持在相对安全稳定的水平。荧光成像结果也显示，与对照相比，CL没有引起生菜叶片的氧化损伤。综上所述，生菜在红蓝连续光照下生长15 d，茎部生物量和抗坏血酸池大小均较大，且叶片未受伤害，从能量角度来看，9 d是最经济有效的增产时间。在连续光照下，抗坏血酸的积累主要和快速受AsA生物合成和氧化相关酶的酶活性水平而不是基因表达水平的调控。
To understand the dynamic changes of lettuce growth, ascorbate pool and metabolism under long-term red and blue continuous light (CL, 24/0 h, 200 μmol·m−2·s−1), growth parameters, ascorbate pool size, as well as activities and gene expression levels of key enzymes involved in the ascorbate metabolism in lettuce leaves were investigated every 3 days during 15 days. In comparison with normal photoperiod (Control, 16/8 h, 200 μmol·m−2·s−1), lettuce plants gained greater shoot fresh weight and dry matter under CL, which depended more on specific leaf weight than leaf area. Both reduced ascorbate (AsA) and dehydroascorbate (DHA) contents were remarkably enhanced by CL and keep a relatively stable level during the experiment. The consistent greater contents of AsA and DHA were respectively attributed to the up-regulation of L-galactono-1,4-lactone dehydrogenase and ascorbate peroxidase activity rather than their gene expression. Although glutathione contents and glutathione reductase activities were elevated by CL, their contribution to AsA regeneration was limited on account of similar activity and expression level of dehydroascorbate reductase with control. In addition, H2O2content was also raised obviously under CL compared with control, but it was maintained at a relative safety and stable level from day 6. Fluorescence imaging results also showed that CL did not induce oxidative damage on lettuce leaves compared with control. It is concluded that lettuce plants grown under red and blue continuous light for 15 days obtained greater shoot biomass and ascorbate pool size without leaf injury, and 9 days was the most cost-effective duration for yield improvement from an energy standpoint. Ascorbate accumulation under continuous light was primarily and rapidly regulated by the enzymes involved in AsA biosynthesis and oxidation at enzymatic activity level rather than gene expression level.
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影响因子：
4.3
作者：
Li, Mingjun;Ma, Fengwang;Liang, Dong
通讯作者：
Liang, Dong
影响因子：
20.5
作者：
A. I. Vélez-Ramírez;W. van Ieperen;D. Vreugdenhil;F. Millenaar
通讯作者：
A. I. Vélez-Ramírez;W. van Ieperen;D. Vreugdenhil;F. Millenaar
影响因子：
6.9
作者：
N. Smirnoff;Glen L. Wheeler
通讯作者：
N. Smirnoff;Glen L. Wheeler
DOI：
--
发表时间：
--
期刊：
Comput. Hum. Behav.
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1071/pp01264
发表时间：
2002-08
期刊：
Functional plant biology : FPB
影响因子：
--
作者：
Chih-Wen Yu;T. Murphy;Wei-Wen Sung;Chin-Ho Lin
通讯作者：
Chih-Wen Yu;T. Murphy;Wei-Wen Sung;Chin-Ho Lin