Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules.
Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules.
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DOI：
10.1371/journal.pone.0131643
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Dickinson ME
中科院分区：
文献类型：
--
作者：
Hsu CW;Poché RA;Saik JE;Ali S;Wang S;Yosef N;Calderon GA;Scott L Jr;Vadakkan TJ;Larina IV;West JL;Dickinson ME
复杂器官的成功工程需要改进的方法来促进人工组织支架的快速和稳定的血管形成。为了实现这一目标，组织工程策略利用生物材料中的促血管生成生长因子单独或联合释放来诱导血管生成。在这项研究中，我们使用活体显微镜来确定体内移植的聚乙二醇二丙烯酸酯水凝胶释放促血管生成因子所引起的关键的、动态的细胞变化。我们的数据显示，与单独使用血管内皮生长因子相比，当使用强大和协同的血管生成因子PDGFBB和FGF2时，巨噬细胞募集到了强大的巨噬细胞，活体成像提示巨噬细胞在内皮末端细胞迁移和吻合以及周细胞样行为中发挥作用。来自体内实验的进一步数据表明，将CSF1与血管内皮生长因子一起输送可以显著改善单独使用血管内皮生长因子时较差的血管生成反应。这些研究表明，将巨噬细胞募集因子纳入促血管生成生物材料支架的设计中，可能是移植人工组织稳定血管形成和存活所必需的关键策略。
Successful engineering of complex organs requires improved methods to promote rapid and stable vascularization of artificial tissue scaffolds. Toward this goal, tissue engineering strategies utilize the release of pro-angiogenic growth factors, alone or in combination, from biomaterials to induce angiogenesis. In this study we have used intravital microscopy to define key, dynamic cellular changes induced by the release of pro-angiogenic factors from polyethylene glycol diacrylate hydrogels transplanted in vivo. Our data show robust macrophage recruitment when the potent and synergistic angiogenic factors, PDGFBB and FGF2 were used as compared with VEGF alone and intravital imaging suggested roles for macrophages in endothelial tip cell migration and anastomosis, as well as pericyte-like behavior. Further data from in vivo experiments show that delivery of CSF1 with VEGF can dramatically improve the poor angiogenic response seen with VEGF alone. These studies show that incorporating macrophage-recruiting factors into the design of pro-angiogenic biomaterial scaffolds is a key strategy likely to be necessary for stable vascularization and survival of implanted artificial tissues.
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DOI：
10.1111/j.1549-8719.2010.00075.x
发表时间：
2011-02
期刊：
Microcirculation (New York, N.Y. : 1994)
影响因子：
--
作者：
Gould DJ;Vadakkan TJ;Poché RA;Dickinson ME
通讯作者：
Dickinson ME
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
20.3
作者：
Fantin, Alessandro;Vieira, Joaquim M.;Ruhrberg, Christiana
通讯作者：
Ruhrberg, Christiana
影响因子：
37.8
作者：
Henry, TD;Annex, BH;McCluskey, ER
通讯作者：
McCluskey, ER
DOI：
10.1111/j.1582-4934.2008.00415.x
发表时间：
2009-08-01
影响因子：
5.3
作者：
Andres, German;Leali, Daria;Presta, Marco
通讯作者：
Presta, Marco