degQ associated with the degS/degU two-component system regulates biofilm formation, antimicrobial metabolite production, and biocontrol activity in Bacillus velezensis DMW1.
degQ associated with the degS/degU two-component system regulates biofilm formation, antimicrobial metabolite production, and biocontrol activity in Bacillus velezensis DMW1.
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DOI：
10.1111/mpp.13389
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发表时间：
2023-12
影响因子：
4.9
通讯作者：
Wu, Huijun
中科院分区：
文献类型：
--
作者：
Yu, Chenjie;Qiao, Junqing;Ali, Qurban;Jiang, Qifan;Song, Yan;Zhu, Linli;Gu, Qin;Borriss, Rainer;Dong, Suomeng;Gao, Xuewen;Wu, Huijun
关键词：
革兰氏阳性细菌Velezensis芽孢杆菌菌株DMW 1产生高水平的抗微生物代谢物，可以抑制植物病原体的生长。我们研究了degQ和degS/degU双组分系统调节DMW 1生防特性的机制。与野生型DMW 1相比，当degQ和degU缺失时，ΔdegQ和ΔdegU的生物膜形成、细胞运动性、定殖活性和抗真菌能力显着降低。生物膜相关基因（epsA、epsB、epsC和tasA）和群集相关基因（swrA和swrB）的表达水平均下调。我们还评估了这两个基因对次级代谢产物的影响。degQ和degU基因减少了表面活性素和大环乳酸素的产生，并上调了芬枯草菌素、伊枯草菌素、芽孢杆菌素和艰难菌素代谢产物的产生。逆转录-定量PCR结果与这些观察结果一致。电泳迁移率变动分析和微量热电泳表明，DegU可以结合到这六个抗菌代谢物基因的启动子区，并调节其合成。总之，我们提供了系统的证据，证明degQ和degU基因是重要的多细胞行为和抗菌代谢过程中的B。velezensis DMW 1的菌株，并提出了用于工业生产抗微生物代谢物的新的顺从菌株。 degQ和degU对于Velezensis DMW 1的生防活性是重要的，并且DegU可以通过靶向其启动子区域来调节次级代谢产物的产生。
The gram‐positive bacterium Bacillus velezensis strain DMW1 produces a high level of antimicrobial metabolites that can suppress the growth of phytopathogens. We investigated the mechanism used by degQ and the degS/degU two‐component system to regulate the biocontrol characteristics of DMW1. When degQ and degU were deleted, the biofilm formation, cell motility, colonization activities, and antifungal abilities of ΔdegQ and ΔdegU were significantly reduced compared to wild‐type DMW1. The expression levels of biofilm‐related genes (epsA, epsB, epsC, and tasA) and swarming‐related genes (swrA and swrB) were all down‐regulated. We also evaluated the impact on secondary metabolites of these two genes. The degQ and degU genes reduced surfactin and macrolactin production and up‐regulated the production of fengycin, iturin, bacillaene, and difficidin metabolites. The reverse transcription‐quantitative PCR results were consistent with these observations. Electrophoretic mobility shift assay and microscale thermophoresis revealed that DegU can bind to the promoter regions of these six antimicrobial metabolite genes and regulate their synthesis. In conclusion, we provided systematic evidence to demonstrate that the degQ and degU genes are important regulators of multicellular behaviour and antimicrobial metabolic processes in B. velezensis DMW1 and suggested novel amenable strains to be used for the industrial production of antimicrobial metabolites. degQ and degU are important for the biocontrol activity of Bacillus velezensis DMW1, and DegU can regulate the production of secondary metabolites by targeting their promoter regions.
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DOI：
10.1016/j.btre.2021.e00686
发表时间：
2021-12
期刊：
Biotechnology reports (Amsterdam, Netherlands)
影响因子：
--
作者：
Rahman FB;Sarkar B;Moni R;Rahman MS
通讯作者：
Rahman MS
影响因子：
4.6
作者：
Ansari, Firoz Ahmad;Ahmad, Iqbal
通讯作者：
Ahmad, Iqbal
解淀粉芽孢杆菌 FZB42 产生的风霉素抑制禾谷镰刀菌生长和霉菌毒素生物合成
影响因子：
4.2
作者：
Hanif, Alvina;Zhang, Feng;Gao, Xuewen
通讯作者：
Gao, Xuewen
影响因子：
3.6
作者：
Kobayashi, Kazuo
通讯作者：
Kobayashi, Kazuo
影响因子：
3.6
作者：
Kearns, DB;Chu, F;Losick, R
通讯作者：
Losick, R