Luffa echinata Roxb. induces human colon cancer cell (HT-29) death by triggering the mitochondrial apoptosis pathway.
Luffa echinata Roxb. induces human colon cancer cell (HT-29) death by triggering the mitochondrial apoptosis pathway.
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丝瓜(Luffa echinata Roxb.)
DOI：
10.3390/molecules17055780
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发表时间：
2012-05-16
期刊：
影响因子：
--
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Shang LH;Li CM;Yang ZY;Che DH;Cao JY;Yu Y
丝瓜果实提取物的抗增殖作用及其诱导细胞死亡机制。(LER)考察LER的甲醇提取物以剂量依赖性和时间依赖性的方式抑制人结肠癌细胞（HT-29）的增殖，并引起凋亡细胞群体的显著增加。此外，LER处理的细胞中观察到明显的单层收缩和破坏，而未处理的细胞中未观察到。不同浓度的LER提取物处理HT-29细胞后，细胞周期分析表明LER提取物通过使细胞周期阻滞于G2/M期来抑制HT-29细胞的增殖。活性氧（ROS）水平的测定表明，LER提取物诱导凋亡细胞死亡通过ROS的产生。此外，LER处理导致线粒体膜电位（MMP）的快速下降，作为荧光的减少。通过RT-PCR分析了几个烟草抗病相关基因的转录本。LER提取物处理后，HT-29细胞caspase-3转录产物明显增多，Bcl-XL mRNA表达水平降低。此外，凋亡相关基因（Bax和Bcl-2）的比率从1.6急剧增加到54.1。这些实验表明，LER具有诱导结肠癌细胞凋亡的抗癌作用，为进一步研究LER对人结肠癌的治疗潜力提供了动力。
The antiproliferative properties and cell death mechanism induced by the extract of the fruits of Luffa echinata Roxb. (LER) were investigated. The methanolic extract of LER inhibited the proliferation of human colon cancer cells (HT-29) in both dose-dependent and time-dependent manners and caused a significant increase in the population of apoptotic cells. In addition, obvious shrinkage and destruction of the monolayer were observed in LER-treated cells, but not in untreated cells. Analysis of the cell cycle after treatment of HT-29 cells with various concentrations indicated that LER extracts inhibited the cellular proliferation of HT-29 cells via G2/M phase arrest of the cell cycle. The Reactive oxygen species (ROS) level determination revealed that LER extracts induced apoptotic cell death via ROS generation. In addition, LER treatment led to a rapid drop in mitochondrial membrane potential (MMP) as a decrease in fluorescence. The transcripts of several apoptosis-related genes were investigated by RT-PCR analysis. The caspase-3 transcripts of HT-29 cells significantly accumulated and the level of Bcl-XL mRNA was decreased after treatment with LER extract. Furthermore, the ratio of mitochondria-dependent apoptosis genes (Bax and Bcl-2) was sharply increased from 1.6 to 54.1. These experiments suggest that LER has anticancer properties via inducing the apoptosis in colon cancer cells, which provided the impetus for further studies on the therapeutic potential of LER against human colon carcinoma.
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影响因子：
8.8
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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