Chromosome clustering by Ki-67 excludes cytoplasm during nuclear assembly.
Chromosome clustering by Ki-67 excludes cytoplasm during nuclear assembly.
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DOI：
10.1038/s41586-020-2672-3
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发表时间：
2020-11
期刊：
影响因子：
64.8
通讯作者：
Gerlich DW
中科院分区：
文献类型：
--
作者：
Cuylen-Haering S;Petrovic M;Hernandez-Armendariz A;Schneider MWG;Samwer M;Blaukopf C;Holt LJ;Gerlich DW
真核生物中复杂的基因调控需要将核转录和RNA加工与胞质翻译有效分离。这种分离是通过核被膜实现的，核被膜控制着大分子通过核孔的交换。然而，在有丝分裂过程中，动物和植物细胞会分解核膜，使细胞质和细胞核成分混合。当核膜重组时，细胞质成分通过受体介导的转运通过核孔从核中移除。然而，这些孔的尺寸限制为39 nm，这就提出了如何从细胞核中清除更大的细胞质成分的问题。在这里，我们表明，大的细胞质成分被取代之前，核膜组装的染色体运动到一个密集的集群。这种聚类发生在染色体接近后期纺锤体的两极时，并由涉及表面活性剂样蛋白Ki-67的微管独立机制介导。Ki-67在有丝分裂的早期阶段形成排斥性分子刷，但在有丝分裂退出期间，刷塌陷，Ki-67促进染色体聚类。我们发现，排除成熟的核糖体从细胞核有丝分裂后依赖于Ki-67调节的染色体聚类。因此，我们的研究表明，染色体力学有助于重建开放有丝分裂后真核细胞的区室化。
Complex gene regulation in eukaryotes requires the effective separation of nuclear transcription and RNA processing from cytosolic translation. This separation is achieved by the nuclear envelope, which controls the exchange of macromolecules through nuclear pores. During mitosis, however, animal and plant cells disassemble the nuclear envelope, allowing cytoplasmic and nuclear components to intermix. When the nuclear envelope is reformed, cytoplasmic components are removed from the nucleus by receptor-mediated transport through nuclear pores. However, these pores have a size limit of 39 nm, raising the question of how larger cytoplasmic components are cleared from the nucleus. Here, we show that large cytoplasmic components are displaced prior to nuclear envelope assembly by movement of chromosomes to a dense cluster. This clustering occurs when chromosomes approach the poles of anaphase spindles and is mediated by a microtubule-independent mechanism that involves the surfactant-like protein Ki-67. Ki-67 forms repulsive molecular brushes during the early stages of mitosis, but during mitotic exit the brushes collapse and Ki-67 promotes chromosome clustering. We show that exclusion of mature ribosomes from the nucleus after mitosis depends on Ki-67-regulated chromosome clustering. Thus, our study reveals that chromosome mechanics help to reestablish the compartmentalization of eukaryotic cells after open mitosis.
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影响因子：
16
作者：
Booth, Daniel G.;Beckett, Alison J.;Molina, Oscar;Samejima, Itaru;Masumoto, Hiroshi;Kouprina, Natalay;Larionov, Vladimir;Prior, Ian A.;Earnshaw, William C.
通讯作者：
Earnshaw, William C.
DOI：
10.1083/jcb.201307172
发表时间：
2014-01-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Tarantino N;Tinevez JY;Crowell EF;Boisson B;Henriques R;Mhlanga M;Agou F;Israël A;Laplantine E
通讯作者：
Laplantine E
影响因子：
16.6
作者：
Lukinavičius G;Blaukopf C;Pershagen E;Schena A;Reymond L;Derivery E;Gonzalez-Gaitan M;D'Este E;Hell SW;Wolfram Gerlich D;Johnsson K
通讯作者：
Johnsson K
影响因子：
16.6
作者：
Eibauer, Matthias;Pellanda, Mauro;Turgay, Yagmur;Dubrovsky, Anna;Wild, Annik;Medalia, Ohad
通讯作者：
Medalia, Ohad
影响因子：
64.8
作者：
Cuylen S;Blaukopf C;Politi AZ;Müller-Reichert T;Neumann B;Poser I;Ellenberg J;Hyman AA;Gerlich DW
通讯作者：
Gerlich DW