Bifurcation of the amphibian embryo's axis: analysis of variation in response to egg centrifugation.
Bifurcation of the amphibian embryo's axis: analysis of variation in response to egg centrifugation.
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两栖动物胚胎轴的分叉：对卵子离心反应的变化分析。
DOI：
10.1387/ijdb.2288862
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
G. Malacinski
中科院分区：
文献类型：
--
作者：
A. Neff;M. Wakahara;G. Malacinski
据报道，爪蟾胚胎对离心的发育反应差异很大。响应离心的变化，作为衡量胚胎存活和孪生的轴向结构，监测不同的产卵非洲爪蟾卵。采用一种方便的方法来量化卵细胞质在离心场中的位移潜力。它涉及到在仔细控制的条件下测试每个产卵的小批量卵，以使细胞质在倒置方向上移动。细胞质不动性（CIM）的值，从而从每个产卵的样品中测量与产卵的发育成功（胚胎存活）和离心后的孪生频率。那些产卵CIM值高（即刚性或僵硬的细胞质）有最高的存活率和最低的频率和严重程度的双胞胎在离心卵。CIM的变化解释了先前在几份报告中指出的对离心反应的广泛变化，并进一步强调了细胞质隔室对卵组织和早期胚胎模式形成的作用。
Xenopus embryos have been reported to vary widely in their developmental response to centrifugation. Variation in response to centrifugation, as measured by embryo survival and twinning of axial structures, was monitored different spawnings of Xenopus laevis eggs. A convenient method for quantifying the egg cytoplasm's potential for displacement in a centrifugal field was employed. It involved testing small batches of eggs from each spawn under carefully controlled conditions for displacement of the cytoplasm while held in an inverted orientation. The cytoplasmic immobility (CIM) values thus measured in samples from each spawn were correlated with the spawning's developmental success (survival of embryos) and the twinning frequency after centrifugation. Those spawnings with high CIM values (i.e. a rigid or stiff cytoplasm) had the highest survival rates and the lowest frequency and severity of twinning in centrifuged eggs. Variations in CIM account for the broad variation in response to centrifugation previously noted in several reports and further emphasize the role cytoplasmic compartments play vis-à-vis egg organization and early embryonic pattern formation.
影响因子：
2.7
作者：
Jean-Paul Vincent;John C. Gerhart
通讯作者：
Jean-Paul Vincent;John C. Gerhart
与青蛙卵的激活和背腹极化相关的聚合微管蛋白水平的变化。
影响因子：
2.7
作者：
Elinson,RP
通讯作者：
Elinson,RP