Changes in levels of polymeric tubulin associated with activation and dorsoventral polarization of the frog egg.
Changes in levels of polymeric tubulin associated with activation and dorsoventral polarization of the frog egg.
复制标题
与青蛙卵的激活和背腹极化相关的聚合微管蛋白水平的变化。
DOI：
10.1016/0012-1606(85)90362-8
复制
发表时间：
1985
影响因子：
2.7
通讯作者：
Elinson,RP
中科院分区：
文献类型：
--
作者：
Elinson,RP
在非洲爪蟾的电激活卵和受精卵的第一个细胞周期中测量聚合微管蛋白的水平。将卵子在微管稳定培养基中均质化，并通过定量蛋白质印迹测定通过离心沉淀的微管蛋白的量。基于沉淀中微管的存在以及冷、诺考达唑和 D2O 的影响，沉淀的微管蛋白（聚合物）呈微管形式。未活化的鸡蛋具有高水平的聚合物（大于 0.1 微克/鸡蛋），该聚合物在活化后几分钟内消失。聚合物的水平保持在较低水平（低于 0.02 μg/蛋），直到细胞周期的一半（标准化时间尺度上为 0.5），此时水平升至预激活值。与中期相关的减少（0.85标准化时间）和在第一次卵裂时恢复到高水平（1.0标准化时间）。受精卵显示出类似的模式，尽管聚合物的量增加较早（0.3-0.5标准化时间），这可能是由于精子大师。激活时微管的解聚表明此时细胞骨架发生了巨大的变化。 0.5 标准化时间的聚合与导致背腹极性的细胞质转移的开始一致。这一结果与之前的抑制剂研究一起表明，微管参与胚胎的背腹极化。
The level of polymeric tubulin was measured during the first cell cycle of the electrically activated and the fertilized egg ofXenopus laevis. Eggs were homogenized in a microtubule-stabilizing medium, and the amount of tubulin pelleted by centrifugation was determined by quantitative Western blots. The pelleted tubulin (polymer) was in the form of microtubules based on the presence of microtubules in the pellet and on the effects of cold, nocodazole, and D2O. Unactivated eggs had a high level of polymer (greater than 0.1 μg/egg) which disappeared within minutes of activation. The level of polymer stayed low (less than 0.02 μg/egg) until halfway through the cell cycle (0.5 on a normalized time scale) when the level rose to the preactivation value. There was a decrease associated with metaphase (0.85 normalized time) and a return to a high level at first cleavage (1.0 normalized time). Fertilized eggs showed a similar pattern although the amount of polymer increased earlier (0.3–0.5 normalized time), presumably due to the spermaster. The depolymerization of microtubules at activation indicates that there is a dramatic change of the cytoskeleton at this time. The polymerization at 0.5 normalized time coincides with the start of the cytoplasmic shift leading to dorsoventral polarity. This result, together with previous inhibitor studies, shows that microtubules are involved in dorsoventral polarization of the embryo.
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DOI：
10.1083/jcb.84.3.668
发表时间：
1980-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Harris P;Osborn M;Weber K
通讯作者：
Weber K
青蛙精子进入的部位和与卵子激活相关的皮质收缩。
影响因子：
2.7
作者：
R. Elinson
通讯作者：
R. Elinson
细胞骨架成分的结构相互作用。
影响因子：
7.8
作者：
Schliwa, M;van Blerkom, J
通讯作者：
van Blerkom, J
影响因子：
2.7
作者：
ELINSON, RP
通讯作者：
ELINSON, RP
人工激活的海胆卵细胞质内聚周期中的微管蛋白动力学。
影响因子：
3.7
作者：
G. Coffe;G. Foucault;M. Raymond;J. Pudles
通讯作者：
J. Pudles