Reprogramming of adult human neural stem cells into induced pluripotent stem cells.
Reprogramming of adult human neural stem cells into induced pluripotent stem cells.
复制标题
将成人神经干细胞重编程为诱导多能干细胞。
DOI：
10.3760/cma.j.issn.0366-6999.20122755
复制
发表时间：
2013
影响因子：
6.1
通讯作者：
Xiaoyuan Feng
中科院分区：
文献类型：
--
作者：
Li;Hua Sun;Tian Wang;Hai;Pu Wang;Jian;Zheng;Xiaoyuan Feng
背景
由于一种从人神经干细胞(HNSCs)获得诱导多能干细胞(IPSCs)的有效方法为我们研究脑疾病提供了一种有前景的工具，我们报道了通过逆转录病毒转导四种确定的因子将成人hNSCs直接重编程为IPSCs。
方法
成功地从癫痫患者的海马区组织中分离和培养了神经干细胞。当结合4种因子(Oct3/4、SOX2、KLF4和c-myc)时，成功地获得了IPSCs克隆。
结果
形态特征和特异的基因表达证实，这些hNSCs来源的IPSCs具有胚胎干细胞样的特性，包括在体外和体内分化为所有三个生殖层的能力。
结论
我们的方法将有助于从神经干细胞中培养出人类IPSCs，并为研究神经疾病提供重要的工具。
BACKGROUND
Since an effective method for generating induced pluripotent stem cells (iPSCs) from human neural stem cells (hNSCs) can offer us a promising tool for studying brain diseases, here we reported direct reprogramming of adult hNSCs into iPSCs by retroviral transduction of four defined factors.
METHODS
NSCs were successfully isolated and cultured from the hippocampus tissue of epilepsy patients. When combined with four factors (OCT3/4, SOX2, KLF4, and c-MYC), iPSCs colonies were successfully obtained.
RESULTS
Morphological characterization and specific genetic expression confirmed that these hNSCs-derived iPSCs showed embryonic stem cells-like properties, which include the ability to differentiate into all three germ layers both in vitro and in vivo.
CONCLUSION
Our method would be useful for generating human iPSCs from NSCs and provide an important tool for studying neurological diseases.
影响因子：
56.9
作者：
Yu, Junying;Vodyanik, Maxim A.;Thomson, James A.
通讯作者：
Thomson, James A.