Indocyanine green-loaded nanoparticles for image-guided tumor surgery.
Indocyanine green-loaded nanoparticles for image-guided tumor surgery.
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DOI：
10.1021/bc5005679
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发表时间：
2015-02-18
影响因子：
4.7
通讯作者：
Mohs, Aaron M.
中科院分区：
文献类型：
--
作者：
Hill, Tanner K.;Abdulahad, Asem;Kelkar, Sneha S.;Marini, Frank C.;Long, Timothy E.;Provenzale, James M.;Mohs, Aaron M.
术中发现阳性肿瘤边缘和局部恶性肿块对患者的长期生存至关重要。使用图像引导手术切除肿瘤，特别是使用近红外荧光成像，是一种潜在的方法，可以方便地切除手术部位的所有肿瘤组织。在这项研究中，我们展示了一系列透明质酸(HL A)衍生的纳米颗粒，它们包裹了近红外染料吲哚青绿，称为NanoICG，用于改善该染料对肿瘤的输送。纳米粒子的自组装是由三个疏水基团中的一个驱动的：氨丙基-1-丙基丁酰胺(PBA)、氨丙基-5-β-胆酰胺(5-βCA)或十八胺(Oda)。对纳米粒子的自组装、染料负载和光学性质进行了表征。纳米ICG显示出猝灭的荧光，可以通过在混合溶剂中分解来激活。在生理相关浓度下，NanoICG被发现是无毒的，暴露也没有发现抑制细胞生长。在建立小鼠移植瘤模型的基础上，利用荧光图像引导手术系统和全动物成像系统，观察到纳米ICG对肿瘤的强烈荧光增强作用。使用NanoICG的肿瘤对比度明显高于单独使用ICG。
Detecting positive tumor margins and local malignant masses during surgery is critical for long-term patient survival. The use of image-guided surgery for tumor removal, particularly with near-infrared fluorescent imaging, is a potential method to facilitate removing all neoplastic tissue at the surgical site. In this study we demonstrate a series of hyaluronic acid (HLA)-derived nanoparticles that entrap the near-infrared dye indocyanine green, termed NanoICG, for improved delivery of the dye to tumors. Self-assembly of the nanoparticles was driven by conjugation of one of three hydrophobic moieties: aminopropyl-1-pyrenebutanamide (PBA), aminopropyl-5β-cholanamide (5βCA), or octadecylamine (ODA). Nanoparticle self-assembly, dye loading, and optical properties were characterized. NanoICG exhibited quenched fluorescence that could be activated by disassembly in a mixed solvent. NanoICG was found to be nontoxic at physiologically relevant concentrations and exposure was not found to inhibit cell growth. Using an MDA-MB-231 tumor xenograft model in mice, strong fluorescence enhancement in tumors was observed with NanoICG using a fluorescence image-guided surgery system and a whole-animal imaging system. Tumor contrast with NanoICG was significantly higher than with ICG alone.
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影响因子：
3.1
作者：
Marshall, Milton V.;Draney, Daniel;Sevick-Muraca, Eva M.;Olive, D. Michael
通讯作者：
Olive, D. Michael
影响因子：
5.8
作者：
Liu, Yanhua;Sun, Jin;He, Zhonggui
通讯作者：
He, Zhonggui
DOI：
10.1016/j.jconrel.2013.10.016
发表时间：
2013-12-28
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Ganesh S;Iyer AK;Gattacceca F;Morrissey DV;Amiji MM
通讯作者：
Amiji MM
影响因子：
14
作者：
Makino, Akira;Kizaka-Kondoh, Shinae;Kimura, Shunsaku
通讯作者：
Kimura, Shunsaku
影响因子：
45.3
作者：
Frangioni, John V.
通讯作者：
Frangioni, John V.