Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia.
Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia.
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DOI：
10.1016/j.scitotenv.2022.159816
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发表时间：
2023-02-01
影响因子：
9.8
通讯作者：
Abe, Shuichi
中科院分区：
文献类型：
--
作者：
Wannigama, Dhammika Leshan;Amarasiri, Mohan;Hongsing, Parichart;Hurst, Cameron;Modchang, Charin;Chadsuthi, Sudarat;Anupong, Suparinthon;Phattharapornjaroen, Phatthranit;Rad, Ali Hosseini S. M.;Fernandez, Stefan;Huang, Angkana T.;Kueakulpattana, Naris;Tanasatitchai, Chanikan;Vatanaprasan, Porames;Saethang, Thammakorn;Luk-in, Sirirat;Storer, Robin James;Ounjai, Puey;Ragupathi, Naveen Kumar Devanga;Kanthawee, Phitsanuruk;Sano, Daisuke;Furukawa, Takashi;Sei, Kazunari;Leelahavanichkul, Asada;Kanjanabuch, Talerngsak;Hirankarn, Nattiya;Higgins, Paul G.;Kicic, Anthony;Chatsuwan, Tanittha;McLellan, Alexander D.;Abe, Shuichi
关键词：
猴痘病毒通过受感染个体的粪便排出。因此，有兴趣在废水中使用病毒载量检测的前哨早期监测在社区一级，并作为一种补充方法，症状监测。我们于2022年5月至8月期间从曼谷市中心的63个有下水道及无下水道的地点收集废水。使用实时聚合酶链反应（PCR）定量猴痘病毒DNA拷贝数，并通过桑格测序确认为阳性。2022年6月第二周首次在废水中检测到猴痘病毒DNA，平均拷贝数为16.4拷贝/ml（n = 3）。从7月的第一周开始，病毒DNA拷贝数增加到平均拷贝数45.92拷贝/ml。对阳性样本进行桑格测序，确认存在猴痘病毒。我们的研究是第一个在泰国不同地点的废水中检测到猴痘病毒DNA的研究。结果表明，这可能是检测病毒DNA和预测即将爆发的补充来源。
The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in wastewater from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks.
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影响因子：
9.8
作者：
de Jonge, Eline F.;Koelewijn, Jaap M.;van der Drift, Anne-Merel R.;Beek, Rudolf F. H. J. van der;Nagelkerke, Erwin;Lodder, Willemijn J.
通讯作者：
Lodder, Willemijn J.
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158.5
作者：
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Miura, Fuminari;van Ewijk, Catharina Else;Backer, Jantien A.;Xiridou, Maria;Franz, Eelco;de Coul, Eline Op;Brandwagt, Diederik;van Cleef, Brigitte;van Rijckevorsel, Gini;Swaan, Corien;van den Hof, Susan;Wallinga, Jacco
通讯作者：
Wallinga, Jacco
影响因子：
56.3
作者：
Adler, Hugh;Gould, Susan;Hine, Paul;Snell, Luke B.;Wong, Waison;Houlihan, Catherine F.;Osborne, Jane C.;Rampling, Tommy;Beadsworth, Mike Bj;Duncan, Christopher Ja;Dunning, Jake;Fletcher, Tom E.;Hunter, Ewan R.;Jacobs, Michael;Khoo, Saye H.;Newsholme, William;Porter, David;Porter, Robert J.;Ratcliffe, Libuse;Schmid, Matthias L.;Semple, Malcolm G.;Tunbridge, Anne J.;Wingfield, Tom;Price, Nicholas M.
通讯作者：
Price, Nicholas M.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro