Transcriptional transitions in Alphonso mango (Mangifera indica L.) during fruit development and ripening explain its distinct aroma and shelf life characteristics.
Transcriptional transitions in Alphonso mango (Mangifera indica L.) during fruit development and ripening explain its distinct aroma and shelf life characteristics.
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DOI：
10.1038/s41598-017-08499-5
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发表时间：
2017-08-18
影响因子：
4.6
通讯作者：
Gupta VS
中科院分区：
文献类型：
--
作者：
Deshpande AB;Anamika K;Jha V;Chidley HG;Oak PS;Kadoo NY;Pujari KH;Giri AP;Gupta VS
阿方索以其独特的风味、诱人的色泽、低纤维的果肉和较长的保质期，被誉为芒果之王。利用Illumina测序技术，从果实发育、成熟和开花的七个阶段对阿方索芒果的转录组进行了分析。这些阶段的总转录组数据在65到143 Mb之间。重要的是，20,755个独特的转录本被注释，4,611个被分配了酶委托编号，它们编码了142条生物途径。这些途径包括与乙烯和风味相关的次生代谢物生物合成途径，以及与淀粉、蔗糖、氨基酸和脂肪酸代谢有关的途径。在果实发育和成熟的不同阶段，数以千计的转录本的差异调节(p值 ≤ 0.05)是明显的。鉴定了参与风味形成的单萜、倍半萜、二萜、内酯和呋喃酮的生物合成的新转录本。大量编码细胞壁修饰酶的转录本被发现稳定表达，而很少有转录本在这些阶段受到差异调控。在阿方索果实发育和成熟过程中同时检测到79个新的细胞壁修饰酶抑制剂的转录本，表明这些酶的活性受控参与果实软化。
Alphonso is known as the “King of mangos” due to its unique flavor, attractive color, low fiber pulp and long shelf life. We analyzed the transcriptome of Alphonso mango through Illumina sequencing from seven stages of fruit development and ripening as well as flower. Total transcriptome data from these stages ranged between 65 and 143 Mb. Importantly, 20,755 unique transcripts were annotated and 4,611 were assigned enzyme commission numbers, which encoded 142 biological pathways. These included ethylene and flavor related secondary metabolite biosynthesis pathways, as well as those involved in metabolism of starch, sucrose, amino acids and fatty acids. Differential regulation (p-value ≤ 0.05) of thousands of transcripts was evident in various stages of fruit development and ripening. Novel transcripts for biosynthesis of mono-terpenes, sesqui-terpenes, di-terpenes, lactones and furanones involved in flavor formation were identified. Large number of transcripts encoding cell wall modifying enzymes was found to be steady in their expression, while few were differentially regulated through these stages. Novel 79 transcripts of inhibitors of cell wall modifying enzymes were simultaneously detected throughout Alphonso fruit development and ripening, suggesting controlled activity of these enzymes involved in fruit softening.
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影响因子：
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通讯作者：
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