Comparative analysis of differentially expressed genes between the ovaries from pregnant and nonpregnant goats using RNA-Seq
Comparative analysis of differentially expressed genes between the ovaries from pregnant and nonpregnant goats using RNA-Seq
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利用 RNA-Seq 比较分析妊娠和非妊娠山羊卵巢差异表达基因
DOI：
10.1186/s40709-019-0095-9
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发表时间：
2019-05
影响因子：
3.3
通讯作者：
Li Wenyong
中科院分区：
文献类型：
--
作者：
Quan Qing;Zheng Qi;Ling Yinghui;Fang Fugui;Chu Mingxing;Zhang Xiaorong;Liu Yong;Li Wenyong
BackgroundA multitude of genes tightly regulate ovarian follicular development and hormone secretion. These complex and coordinated biological processes are altered during pregnancy. In order to further understand the regulatory role of these genes during pregnancy, it is important to screen the differentially expressed genes (DEGs) in the ovaries of pregnant and nonpregnant mammals. To detect the genes associated with the development of pregnancy in goats, DEGs from the ovaries from pregnant and nonpregnant Anhui white goats (pAWGs and nAWGs, respectively) were analyzed using RNA sequencing technology (RNA-Seq).ResultsIn this study, 13,676,394 and 13,549,560 clean reads were generated from pAWGs and nAWGs, respectively, and 1724 DEGs were identified between the two libraries. Compared with nAWGs, 1033 genes were upregulated and 691 genes were downregulated in pAWGs, includingPGR,PRLR,STARandCYP19A1, which play important roles in goat reproduction. Gene Ontology analysis showed that the DEGs were enriched for 49 functional GO terms. Kyoto Encyclopedia of Genes and Genomes analysis revealed that 397 DEGs were significantly enriched in 13 pathways, including “cell cycle”, “cytokine–cytokine receptor interaction” and “steroid biosynthesis”, suggesting that the genes may be associated with cell cycle regulation, follicular development and hormone secretion to regulate the reproduction process. Additionally, quantitative real-time PCR was used to verify the reliability of the RNA-Seq data.ConclusionsThe data obtained in this work enrich the genetic resources of goat and provide a further understanding of the complex molecular regulatory mechanisms occurring during the development of pregnancy and reproduction in goats. The DEGs screened in this study may play an important role in follicular development and hormone secretion and they would provide scientific basis for related research in the future.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
利用RNA-Seq技术研究齐拉黑羊和和田羊卵巢中的差异基因表达。
影响因子：
3.7
作者：
Chen HY;Shen H;Jia B;Zhang YS;Wang XH;Zeng XC
通讯作者：
Zeng XC
影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
影响因子：
4.8
作者：
Chakravarthi, V. Praveen;Khristi, Vincentaben;Rumi, M. A. Karim
通讯作者：
Rumi, M. A. Karim
DOI：
10.1126/science.1233321
发表时间：
2013-03-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fung KY;Mangan NE;Cumming H;Horvat JC;Mayall JR;Stifter SA;De Weerd N;Roisman LC;Rossjohn J;Robertson SA;Schjenken JE;Parker B;Gargett CE;Nguyen HP;Carr DJ;Hansbro PM;Hertzog PJ
通讯作者：
Hertzog PJ