Multiple viral respiratory pathogens in children with bronchiolitis.
Multiple viral respiratory pathogens in children with bronchiolitis.
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细支气管炎儿童的多种病毒呼吸道病原体。
DOI：
10.1111/j.1651-2227.2008.01023.x
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发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Zerr DM
中科院分区：
文献类型：
--
作者：
Stempel HE;Martin ET;Kuypers J;Englund JA;Zerr DM
目的：本研究的目的是描述从在儿童医院接受治疗的毛细支气管炎幼儿中获得的鼻标本中病毒病原体的频率和合并感染的相对频率。研究方法：我们使用真实的时间PCR和荧光抗体检测结果对小于2岁且ICD-9-CM编码为毛细支气管炎的儿童的鼻洗液标本进行了研究。所有标本均在2003年10月至2004年4月的呼吸季节期间在美国华盛顿州西雅图的儿童医院采集用于临床护理。结果：180例毛细支气管炎患儿中168例检出病毒，检出率为93%。127名儿童（71%）被发现携带单一病毒，41名儿童（23%）被发现携带多种病毒。呼吸道合胞病毒（RSV）是最常见的病毒（77%），其次是腺病毒（15%）、人偏肺病毒（11%）、冠状病毒（8%）、副流感病毒（6%）和流感病毒（1%）。在检出RSV的139份样本中，34份（24%）合并感染了另一种病毒病原体。结论：分子诊断技术在评估为毛细支气管炎的儿童中发现了高频率的病毒和病毒合并感染。有必要进一步研究RSV以外的病毒病原体和RSV合并感染在毛细支气管炎儿童中的作用。
Aim: The aim of the study was to describe the frequency of viral pathogens and relative frequency of co‐infections in nasal specimens obtained from young children with bronchiolitis receiving care at a children's hospital. Methods: We conducted a study of nasal wash specimens using real‐time PCR and fluorescent‐antibody assay results from children less than two with an ICD‐9‐CM code for bronchiolitis. All specimens were collected for clinical care at Children's Hospital in Seattle, WA, USA, during the respiratory season from October 2003 to April 2004. Results: Viruses were detected in 168 (93%) of the 180 children with bronchiolitis. A single virus was identified in 127 (71%) children and multiple viruses in 41 (23%). Respiratory syncytial virus (RSV) was the most common virus detected (77%), followed by adenovirus (15%), human metapneumovirus (11%), coronavirus (8%), parainfluenza (6%) and influenza (1%). Of the 139 samples with RSV detected, 34 (24%) were co‐infected with another viral pathogen. Conclusion: Molecular diagnostic techniques identified a high frequency of viruses and viral co‐infections among children evaluated for bronchiolitis. Further study of the role of viral pathogens other than RSV and co‐infections with RSV in children with bronchiolitis appears warranted.
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DOI：
10.1016/j.jcv.2004.03.018
发表时间：
2004-10
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
Kuypers J;Wright N;Morrow R
通讯作者：
Morrow R
影响因子：
8
作者：
Yorita, Krista L.;Holman, Robert C.;Schonberger, Lawrence B.
通讯作者：
Schonberger, Lawrence B.
影响因子：
9.4
作者：
Kuypers, Jane;Wright, Nancy;Morrow, Rhoda
通讯作者：
Morrow, Rhoda
影响因子：
6.9
作者：
Ong, GM;Wyatt, DE;Coyle, PV
通讯作者：
Coyle, PV
影响因子：
11.8
作者：
Greensill J;McNamara PS;Dove W;Flanagan B;Smyth RL;Hart CA
通讯作者：
Hart CA